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ISSN 在线: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Influence of Selected Microelements on the Laccase and Peroxidases Production by Pleurotus eryngii (DC.: Fr.) Quel. in Submerged Cultures

卷 7, 册 3, 2005, 470 pages
DOI: 10.1615/IntJMedMushrooms.v7.i3.1010
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摘要

Pleurotus eryngii var. eryngii is a white-rot fungus because of its ability to produce extracellular ligninolytic enzymes laccase (Lac), two peroxidases (Mn dependent peroxidase, MnP, and versatile peroxidase, VP), and aryl-alcohol oxidase (AAO). Microelements copper (Cu), manganese (Mn), zinc (Zn), iron (Fe), and selenium (Se) influenced the Lac and peroxidase production by this species.
The aim of this investigation was to study the effect of Cu, Mn, Fe, Zn, and Se under conditions of submerged fermentation (SF) of dry ground mandarin peels on the production of Lac and peroxidases in the selected P. eryngii var. eryngii strain.
The culture of P. eryngii var. eryngii, strain No. 616, is preserved in the Culture Collection of the Institute of Evolution, University of Haifa (HAI). The investigated P. eryngii strain was grown under SF conditions of dry ground mandarin peels (as a carbon source) and (NH4)2SO4 (as a nitrogen source). Different combinations and concentrations of Cu, Mn, Fe, Zn, and Se in the forms of: CuSO4 × 7H2O, MnSO4 × H2O, FeSO4 × 7H2O, ZnSO4 × 7H2O, Na2SeO3, Na2SeO4, and SeO2 were analyzed. Ligninolytic enzyme activities were measured after 5 and 7 days of cultivation. Lac and peroxidase activities were determined using syringaldasine and phenol red, respectively. An UV-160A Spectrophotometer (Shimaden) was used for these assays.
The optimum Cu2+ concentration for Lac production was 1 mM (300.12 U/L after 7 days of cultivation), while for peroxidases 10 mM Mn2+ concentration of 5 mM led to peaks of Lac (322.62 U/L, after 7 days of cultivation) and peroxidase activities. However, peroxidase production was significantly lower at Mn2+ concentrations of 1 mM and 3 mM compared to the control. The addition of Zn2+ and Fe2+ to the medium in a concentration of 1 mM led to the significantly high increase of Lac, slight increase of VP, and decrease of MnP activity. Se presented in the concentration of 1 mM, in all investigated forms, caused a decrease of Lac activity, especially when it was added in the form of SeO2, while the lowest decrease was shown in the presence of Na2SeO4. In the presence of all forms of Se, MnP activity decreased after 5 days and increased after 7 days of cultivation in comparison with the control, while VP activity increased, especially when Se was presented in the form of SeO2.

对本文的引用
  1. Dosdall Rovena, Preuß Franziska, Hahn Veronika, Schlüter Rabea, Schauer Frieder, Decay of the water reed Phragmites communis caused by the white-rot fungus Phlebia tremellosa and the influence of some environmental factors, Applied Microbiology and Biotechnology, 102, 1, 2018. Crossref

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