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ISSN 打印: 1521-9429

ISSN 在线: 1940-4328

SJR: 0.168 SNIP: 0.377 CiteScore™:: 0.6 H-Index: 11

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Morphological and Molecular Characterization of the Representative of Brasilonema (Scytonemataceae, Cyanoprokaryota) from the Tropical Greenhouse in Kiev (Ukraine)

卷 22, 册 2, 2020, pp. 103-122
DOI: 10.1615/InterJAlgae.v22.i2.10
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摘要

This paper presents morphological and molecular characterizations of the representative of the pantropic genus Brasilonema Fiore et al. first discovered in a European greenhouse. The genus described in 2007, using an integrated approach, is morphologically close to Scytonema Agardh; differing in the filaments forming ascending bundles, rarely occurring false branching, and the color of cells. To date, 12 species of Brasilonema have been described; all of them occur subaerophytically in humid tropical and subtropical regions. For several years in the greenhouse of the N.N. Grishko National Botanical Garden of the NAS of Ukraine, we observed abundant blackish mats with a fleecy surface, formed by falsely branching filaments of heterocytous cyanobacteria lilac, pale purple or gray in color. Cyanobacteria densely covered the vegetative organs of various tropical epiphytic plants from the Orchidaceae and Bromeliaceae families; sometimes inhabiting concrete and wooden substrates in the greenhouse. The morphological features of this newly discovered cyanobacteria were studied both in natural material greenhouse samples and culture. For cultivation, liquid and agarized N-free BG11 medium was used. The specimens from the mats and the cultures had some differences in thalli habitus, coloration and arrangement of filaments, frequency of false branching, trichome appearance, dimensional limits, etc. A comparative analysis of the original data with descriptions of the known Brasilonema species showed that the Kiev population coincides morphologically and ecologically with several species, of which it's closest relative is B. octagenarum Aguiar et al. Phylogenetic analysis of the nucleotide sequence of the gene encoding 16S rRNA confirmed affiliation of the original strains to Brasilonema. Analysis of the nucleotide sequence of the 16S-23S ITS region, as well as the secondary structure of its most informative helices, showed the closest proximity of the Kiev material to B. octagenarum, which in turn is probably a complex of species whose taxonomic separation is possible in the future. Presumably, cyanobacteria have entered into the Kiev greenhouse with tropical plants, brought by Ukrainian botanists from an expedition to Brazil in 1986.

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对本文的引用
  1. Jung Patrick, D’Agostino Paul M., Büdel Burkhard, Lakatos Michael, Symphyonema bifilamentata sp. nov., the Right Fischerella ambigua 108b: Half a Decade of Research on Taxonomy and Bioactive Compounds in New Light, Microorganisms, 9, 4, 2021. Crossref

  2. Renaudin Marie, Laforest-Lapointe Isabelle, Bellenger Jean-Philippe, Unraveling global and diazotrophic bacteriomes of boreal forest floor feather mosses and their environmental drivers at the ecosystem and at the plant scale in North America, Science of The Total Environment, 837, 2022. Crossref

  3. Barbosa Maximiliano, Berthold David E., Lefler Forrest W., Dail Laughinghouse IV H., Diversity of the genus Brasilonema (Nostocales, Cyanobacteria) in plant nurseries of central Florida (USA) with the description of three new species: B. fioreae sp. nov., B. santannae sp. nov. and B. wernerae sp. nov., Fottea, 21, 1, 2021. Crossref

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