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ISSN Druckformat: 1947-5764
ISSN Online: 1947-5772
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Viability and Cell Biology for HeLa and Vero Cells after Exposure to Low-Temperature Air Dielectric Barrier Discharge Plasma
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ABSTRAKT
Cancer study is among the hottest topics in plasma medicine. Differential killing of cancer cells and controllable selectivity may be achieved using exposure to plasma generated by various atmospheric pressure discharges. In this article, we discuss the possibility of generating low-temperature air plasma directly inside standard 24- and 96-well cell-culture plates and its subsequent use to achieve selective cytotoxicity. After exposure, cell viability was assessed and molecular biology tests carried out to understand the effect of plasma on cell biology. HeLa (neoplastic cells) and Vero (normal cells) cultures were exposed to air dielectric barrier discharge plasma. We performed viability assessment using the 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide assay at 8 and 24 hr after treatment and found a reduction in cell viability, with a variable amplitude of effect. The cytotoxicity of plasma on HeLa cells at 8 hr was 59.92% and reached 68.58% by 24 hr. The cytotoxic effect on Vero cells was limited to 37.75% at 8 hr and only 6.33% at 24 hr. General cell biology analyses were assessed using flow cytometry and gene expression tracking.
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