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International Journal of Medicinal Mushrooms

Erscheint 12 Ausgaben pro Jahr

ISSN Druckformat: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

In vitro Synthesis of a Recombinant Fungal Immunomodulatory Protein from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W.Curt.:Fr.) P.Karst. (Aphyllophoromycetideae) and Analysis of Its Immunomodulatory Activity

Volumen 12, Ausgabe 4, 2010, pp. 347-358
DOI: 10.1615/IntJMedMushr.v12.i4.20
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ABSTRAKT

The FIP-glu (Lingzhi-8 or LZ-8) isolated from medicinal mushroom Ganoderma lucidum (Lingzhi or Reishi) was the first identified and characterized fungal immunomodulatory protein (FIP), and its biological functions have been explored extensively. On the basis of cloned LZ-8 gene sequence from the genomic DNA of G. lucidum, we expressed FIP-glu by the expression cassette vector pQE-30. The recombinant protein was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Matrix-assisted laser desorption/ ionization mass spectrometry (MALDI-MS). Finally, its bioactivity was examined by inducing expression of cytokine genes in mouse spleen cells. The results showed that recombinant FIP-glu protein could be expressed in E. coli successfully. The yield of recombinant FIP-glu accounted for 51.1% of the total protein of E. coli, while soluble recombinant FIP-glu accounted for 55.4% of the total soluble protein of E. coli. The purity of recombinant FIP-glu protein was more than 90% after purification. Analysis of RT-PCR demonstrated that the recombinant FIP-glu could enhance the transcription of interleukin (IL)-2, IL-3, IL-4, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-2 receptor (IL-2R) genes in mouse spleen cells.

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