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International Journal of Medicinal Mushrooms

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ISSN Druckformat: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Mating System and Genetic Variations of Tricholoma crassum (Berk.) Sacc. in Some Area of Thailand by Isozyme Electrophoresis and PCR-RFLP Method

Volumen 7, Ausgabe 3, 2005, 475 pages
DOI: 10.1615/IntJMedMushrooms.v7.i3.1050
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ABSTRAKT

Tricholoma crassum (Tricholomataceae, Agaricales) is a large, good edible species. It has nutritional quality, having 10.02 gm carbohydrate, 0.287 gm fats, 18.58 gm protein, 2.71 mg calcium, and 3.35 mg iron in every 100 gm of fresh weight. It is found growing abundantly throughout Thailand.
The mating system of T. crassum was studied. The monokaryon isolates were selected from ten sporocarp collections in five provinces in Thailand—Ubonratchathani, Sakonnakorn, Mahasarakham, Srisaket, and Nakornratcha-sima. Suitable growth conditions for the mycelial cultivation were PDYA medium pH 7 and 25 °C incubation. Twelve fast-growing monokaryotic strains were selected for mating system studies. The mating system was determined by pairing the monokaryotic mycelia of each collection in all pairwise combinations. The presence of clamp connections after mating indicated sexual compatibility. The ratio of compatible crosses to all combination crosses were 1:4, which indicated tetrapolar heterothallism. In addition, multiple alleles among the monokaryons for each sporocarp of the five provinces were examined on the basis of their mating interactions, and the results were two factors (A and B), and each possessed 16 different alleles.
One hundred and thirty-eight monokarytic isolates of T. crassum from the above five provinces were cultured on growth media. The suitable conditions for the mycelial growth were PDYB medium at 25 °C for 21 days. All samples were analyzed for isozyme variability on polyacrylamide gel electrophoresis with 11 enzyme systems: isocitrate dehydrogenase, leucine aminopeptidase, acid phosphatase, phosphogluconate dehydrogenase, alkaline phosphatase, alcohol dehydrogenase, glucose− 6−phosphate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, laccase, and esterase. Eight of the systems showed polymorphism. Cluster analysis based on isozyme variability using the NTSYSpc 2.00 and UPGMA methods revealed two clusters at a similarity coefficient of 0.67. The first cluster consisted of monokaryotic isolates from Nakornratchasima, Mahasalakham, and eight samples of Ubonratchathani. The second cluster consisted of the isolates from Sakonnakhorn, Srisaket, and 30 samples of Ubonratchathani.
The genetic variations of nine additional samples of T. crassum from four additional provinces—Roiyed, Burerum, Patumthani, and Nakhon Pathom—were studied by the technique of PCR-RFLP. Two pairs of primers, ITS1- ITS4 and O1-LR12R, were used respectively for PCR amplification on ITS (internal transcribed spacer) and IGS (intergenic spacer) regions of the nuclear ribosomal gene, followed by digestion with HindIII, DdeI, HaeIII, EcoRI, and HinfI. Data analyses of the PCR-RFLP products based on the similarity index and the UPGMA method in the WinBoot program revealed three clusters that were related to their geographic origins, except the samples from Burirum, which showed genetic variation from the same areas at a similarity coefficient of 0.8 and were grouped into the third cluster.

REFERENZIERT VON
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  2. Patra Pradip, Bhanja Sunil K., Sen Ipsita K., Nandi Ashis K., Samanta Surajit, Das Debsankar, Devi K. Sanjana P., Maiti Tapas K., Acharya Krishnendu, Islam Syed S., Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc, Carbohydrate Research, 362, 2012. Crossref

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