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ISSN Druckformat: 1521-9437
ISSN Online: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
Indexed in
Molecular Identification of Some Medicinally Important Aphyllophorales Mushrooms Based on ITS rDNA Sequences and RAPD Data
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ABSTRAKT
Analysis of genetic relationship of important Aphyllophorales germplasm accessions of the National Research Centre for Mushroom, Solan, India, was conducted using RAPD and ITS rDNA sequences. RAPD profiles exhibited significant variations among Trametes versicolor, Tyromyces chioneus, and Ganoderma species. The resulting RAPD-based phylogram grouped Ganoderma spp. accessions into four clades. Phylogeny inferred from ITS rDNA sequence distinguished Trametes versicolor, Tyromyces chioneus, and Ganoderma spp. at the generic level due to different lengths and variations of the 5.8S rDNA conserved region. At the intrageneric level, Ganoderma accessions were divided into two distinct groups based on nucleotide substitution in ITS-1 and ITS-2 regions. It is presumed that some of the Ganoderma sp. samples might be G. lucidum. Single nucleotide substitutions turned out to be more useful for genetic cataloguing of isolates within a species than sequence alignment using NCBI databse.
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Singh S. K., Doshi Anila, Pancholy Anjly, Pathak Rakesh, Biodiversity in wood-decay macro-fungi associated with declining arid zone trees of India as revealed by nuclear rDNA analysis, European Journal of Plant Pathology, 136, 2, 2013. Crossref
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Rolim Leonardo do Nascimento, Cavalcante Maria Auxiliadora de Queiroz, Urben Arailde Fontes, Buso Glaucia Salles Cortopassi, Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains, Brazilian Archives of Biology and Technology, 54, 2, 2011. Crossref