Published 4 issues per year
ISSN Print: 0731-8898
ISSN Online: 2162-6537
Indexed in
Role of Cannabinoid and Vanilloid Receptors in Invasion of Human Breast Carcinoma Cells
ABSTRACT
It is known that the diversified effects of cannabinoid on the fate of carcinoma cells are mediated predominantly through receptors. However, little is known about the effects of the individual activities of cannabinoid and noncannabinoid receptors. Here we investigate the role of cannabinoid receptor (CB) 1, CB2, and transient receptor potential vanilloid type 1 in cell proliferation and invasion patterns in the MDA-MB-231 cell line.
Our results showed that activation of CB1 and vanilloid receptors by methanandamide, a nonselective agonist, and arachidonyl-2'-choloroethylamide (ACEA) and N-oleoyldopamine, selective agonists, reduced invasion of MDA-MB-231 cells at pharmacological concentrations. Accordingly, CB1 activation resulted in decreased expression of matrix metalloproteinase (MMP) 2. On the other hand, administration of a CB2 agonist (CB65) increased cell invasion and expression of MMP2. The data obtained from MTT assay did not show any correlation between reduced invasion and cytotoxic effects of drugs. In addition, the level of vascular endothelial growth factor was significantly reduced in treatment with (R)-(+)-methanandamide, ACEA, CB65, and AM251 (a potent agonist for GPR55 and selective antagonist of CB1) compared with control. Elevated expression of cyclooxygenase-2 was observed in all of the MDA-MB-231 cells treated with agonists.
These results underline the influence of cannabinoid and vanilloid receptors on the invasiveness of MDA-MB-231 human breast carcinoma cells.
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Ramer Robert, Hinz Burkhard, , 314, 2015. Crossref
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dos Santos Daiane S., Piovesan Luciana A., D’Oca Caroline R. Montes, Hack Carolina R. Lopes, Treptow Tamara G.M., Rodrigues Marieli O., Vendramini-Costa Débora B., Ruiz Ana Lucia T.G., de Carvalho João Ernesto, D’Oca Marcelo G. Montes, Antiproliferative activity of synthetic fatty acid amides from renewable resources, Bioorganic & Medicinal Chemistry, 23, 2, 2015. Crossref
-
Ayakannu Thangesweran, Taylor Anthony H., Willets Jonathan M., Konje Justin C., The evolving role of the endocannabinoid system in gynaecological cancer, Human Reproduction Update, 21, 4, 2015. Crossref
-
Javid Farideh A., Phillips Roger M., Afshinjavid S., Verde Roberta, Ligresti Alessia, Cannabinoid pharmacology in cancer research: A new hope for cancer patients?, European Journal of Pharmacology, 775, 2016. Crossref
-
Ramer Robert, Hinz Burkhard, Antitumorigenic targets of cannabinoids – current status and implications, Expert Opinion on Therapeutic Targets, 20, 10, 2016. Crossref
-
Ramer Robert, Hinz Burkhard, Cannabinoids as Anticancer Drugs, in Cannabinoid Pharmacology, 80, 2017. Crossref
-
Freund Patricia, Porpaczy Edit A., Le Trang, Gruber Michaela, Pausz Clemens, Staber Philipp, Jäger Ulrich, Vanura Katrina, Velasco Guillermo, Cannabinoid Receptors Are Overexpressed in CLL but of Limited Potential for Therapeutic Exploitation, PLOS ONE, 11, 6, 2016. Crossref
-
Mir Manzoor A., Hamdani Syed S., Sheikh Bashir A., Mehraj Umar, Recent Advances in Metabolites from Medicinal Plants in Cancer Prevention and Treatment, Current Immunology Reviews, 15, 2, 2019. Crossref
-
Chakravarti Bandana, Ravi Janani, Ganju Ramesh K., Cannabinoids as therapeutic agents in cancer: current status and future implications, Oncotarget, 5, 15, 2014. Crossref
-
He Dan, Wang Jun, Zhang Chunfang, Shan Bin, Deng Xiyun, Li Bin, Zhou Yanwu, Chen Wei, Hong Jidong, Gao Yang, Chen Zhuchu, Duan Chaojun, Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer, Molecular Cancer, 14, 1, 2015. Crossref
-
Susanna Tojkander Sari, TRPV Family Ion Channels in the Mammary Epithelium: Role in Normal Tissue Homeostasis and along Breast Cancer Progression, in Ion Channels - From Basic Properties to Medical Treatment [Working Title], 2022. Crossref