Published 4 issues per year
ISSN Print: 1947-5764
ISSN Online: 1947-5772
Indexed in
The Use of an Atmospheric Pressure Plasma Jet to Inhibit Common Wound-Related Pathogenic Strains of Bacteria
ABSTRACT
The Plaz4 electrosurgical generator produces a nonequilibrium atmospheric pressure plasma jet (APPJ) at 26°C. This APPJ was tested for its antibacterial capabilities on common wound-related pathogens. The inhibition zone (IZ) of bacterial growth and surviving colony-forming units (CFUs) within the IZ were determined for 4 common clinical isolates: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. Agar was inoculated at a high density with a bacterial culture medium (107 CFUs/mL). The strains' susceptibility to the APPJ was tested at exposure times between 30 and 140 seconds. A positive correlation between plasma exposure time and bacterial growth IZ size, and a negative correlation between exposure time and the number of surviving CFUs inside the IZ, were observed. It was possible to achieve a round IZ, 4 cm in diameter, for all strains after 70 seconds of exposure, with less than 10 CFUs/cm2 surviving within the zone. P. aeruginosa was more resistant to plasma and required a longer exposure to achieve an IZ similar to that of other strains. However, the number of CFUs surviving inside the IZ was smaller for this strain for a majority of test conditions. Intraoperative contamination is a significant cause of perioperative infection. Drug-resistant bacteria are endemic to hospitals and are a significant public health concern. With the increasing risk of infections related to drug-resistant bacteria, it is crucial to look for alternative treatments. Electrosurgical generators are routinely used in surgical cases. Therefore, Plaz4 applied to surgical sites or debrided wounds could result in a clinically meaningful reduction in tissue bioburden.
-
Dai Xiaofeng, Bazaka Kateryna, Richard Derek J., Thompson Erik (Rik) W., Ostrikov Kostya (Ken), The Emerging Role of Gas Plasma in Oncotherapy, Trends in Biotechnology, 36, 11, 2018. Crossref
-
Kramer Axel, Schauer Frieder, Papke Roald, Bekeschus Sander, Plasma Application for Hygienic Purposes in Medicine, Industry, and Biotechnology: Update 2017, in Comprehensive Clinical Plasma Medicine, 2018. Crossref
-
Yan Dayun, Malyavko Alisa, Wang Qihui, Ostrikov Kostya (Ken), Sherman Jonathan H., Keidar Michael, Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism, Biomedicines, 9, 9, 2021. Crossref
-
Dai Xiaofeng, Li Jitian, Chen Yiming, Ostrikov Kostya (Ken), When Onco-Immunotherapy Meets Cold Atmospheric Plasma: Implications on CAR-T Therapies, Frontiers in Oncology, 12, 2022. Crossref
-
Trimukhe Ajinkya M., Pandiyaraj K. N., Patekar Mukesh, Miller Vandana, Deshmukh Rajendra R., Perspectives and Advances of Nonthermal Plasma Technology in Cancers, IEEE Transactions on Plasma Science, 50, 8, 2022. Crossref