Library Subscription: Guest
Plasma Medicine

Published 4 issues per year

ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

Indexed in

The Use of an Atmospheric Pressure Plasma Jet to Inhibit Common Wound-Related Pathogenic Strains of Bacteria

Volume 6, Issue 1, 2016, pp. 1-12
DOI: 10.1615/PlasmaMed.2016015851
Get accessDownload

ABSTRACT

The Plaz4 electrosurgical generator produces a nonequilibrium atmospheric pressure plasma jet (APPJ) at 26°C. This APPJ was tested for its antibacterial capabilities on common wound-related pathogens. The inhibition zone (IZ) of bacterial growth and surviving colony-forming units (CFUs) within the IZ were determined for 4 common clinical isolates: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. Agar was inoculated at a high density with a bacterial culture medium (107 CFUs/mL). The strains' susceptibility to the APPJ was tested at exposure times between 30 and 140 seconds. A positive correlation between plasma exposure time and bacterial growth IZ size, and a negative correlation between exposure time and the number of surviving CFUs inside the IZ, were observed. It was possible to achieve a round IZ, 4 cm in diameter, for all strains after 70 seconds of exposure, with less than 10 CFUs/cm2 surviving within the zone. P. aeruginosa was more resistant to plasma and required a longer exposure to achieve an IZ similar to that of other strains. However, the number of CFUs surviving inside the IZ was smaller for this strain for a majority of test conditions. Intraoperative contamination is a significant cause of perioperative infection. Drug-resistant bacteria are endemic to hospitals and are a significant public health concern. With the increasing risk of infections related to drug-resistant bacteria, it is crucial to look for alternative treatments. Electrosurgical generators are routinely used in surgical cases. Therefore, Plaz4 applied to surgical sites or debrided wounds could result in a clinically meaningful reduction in tissue bioburden.

CITED BY
  1. Dai Xiaofeng, Bazaka Kateryna, Richard Derek J., Thompson Erik (Rik) W., Ostrikov Kostya (Ken), The Emerging Role of Gas Plasma in Oncotherapy, Trends in Biotechnology, 36, 11, 2018. Crossref

  2. Kramer Axel, Schauer Frieder, Papke Roald, Bekeschus Sander, Plasma Application for Hygienic Purposes in Medicine, Industry, and Biotechnology: Update 2017, in Comprehensive Clinical Plasma Medicine, 2018. Crossref

  3. Yan Dayun, Malyavko Alisa, Wang Qihui, Ostrikov Kostya (Ken), Sherman Jonathan H., Keidar Michael, Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism, Biomedicines, 9, 9, 2021. Crossref

  4. Dai Xiaofeng, Li Jitian, Chen Yiming, Ostrikov Kostya (Ken), When Onco-Immunotherapy Meets Cold Atmospheric Plasma: Implications on CAR-T Therapies, Frontiers in Oncology, 12, 2022. Crossref

  5. Trimukhe Ajinkya M., Pandiyaraj K. N., Patekar Mukesh, Miller Vandana, Deshmukh Rajendra R., Perspectives and Advances of Nonthermal Plasma Technology in Cancers, IEEE Transactions on Plasma Science, 50, 8, 2022. Crossref

780 Article views 1531 Article downloads Metrics
780 VIEWS 1531 DOWNLOADS 5 Crossref CITATIONS Google
Scholar
CITATIONS

Articles with similar content:

Accelerated Healing of Chronic Wounds under a Combinatorial Therapeutic Regimen Based on Cold Atmospheric Plasma Jet Using Contact and Noncontact Styles Plasma Medicine, Vol.11, 2021, issue 2
Defi Nurul Hayati, Nasruddin Nasruddin, Sri Darmawati, Eka Sakti Wahyuningtyas, Toshio Nakatani, Putri Kurniasiwi, Tatsuo Ishijima, Gela Setya Ayu Putri, Laela Hayu Nurani, Arya Iswara, Junko Sugama
TNF-α Expression and New Epithelial Thickness in the Skin of Mice (Mus musculus) Infected MRSA by Medical Plasma Treatment Plasma Medicine, Vol.12, 2022, issue 4
Defi Nurul Hayati, Sri Darmawati, Gela Setya Ayu Putri, Mudyawati Kamarudin
Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for Pseudomonas aeruginosa Biofilm Eradication Plasma Medicine, Vol.7, 2017, issue 1
Diana Grondona, Leandro Giuliani, Juliana Soler-Arango, Magali Xaubet, Graciela Brelles-Mariño
Bactericidal Efficacy of Dielectric Barrier Discharge Plasma on Methicillin-Resistant Staphylococcus aureus and Escherichia coli in Planktonic Phase and Colonies In vitro Plasma Medicine, Vol.5, 2015, issue 1
Halim Ayan, Niloofar Sanaei
A Pilot Study of Atmospheric Nonthermal Plasma Jet Application on Staphylococcus aureus and Staphylococcus epidermidis Plasma Medicine, Vol.2, 2012, issue 4
Jilu Shen, Chunjun Yang, Cheng Cheng, Shengxiu Liu, Wenhui Du, Shi Chen, Longdan Liu, Shumei Zhang, Jing Gao, Jie Shen, Shenghai Huang
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain