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Plasma Medicine

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ISSN Print: 1947-5764

ISSN Online: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Differential Viability of Eight Human Blood Mononuclear Cell Subpopulations After Plasma Treatment

Volume 3, Issue 1-2, 2013, pp. 1-13
DOI: 10.1615/PlasmaMed.2014008450
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ABSTRACT

In plasma medicine, basic and translational research aids in future application of cold plasma sources in human diseases or disorders (e.g., chronic wounds). While most work has focussed on the interaction of skin cells with plasma, immune system cells have only been marginally examined. Their role is of major importance because they fulfill key regulatory parts in immune responses and modulate inflammation in all types of tissues. This work systematically investigates eight different subpopulations (monocytes and CD4+, CD8+, B, NK, NKT, TH17, and γδ T cells) of human peripheral blood mononuclear cells with regard to viability after 5, 20, or 60 s of plasma treatment. Twenty-four hours after exposure, viability differed between populations (23.1% CD4+ versus 41.9% γδ T cells after 60 s of exposure) as revealed by flow cytometry. Cellular activation before plasma treatment increased survival in all subpopulations tested (26.8% in nonstimulated versus 50.0% in stimulated CD8+ T cells after 60 s of exposure). All lymphocyte subpopulations showed significantly (P < 0.05) lower survival rates compared to monocytes (35.9% for B cells versus 82.5% for monocytes after 60 s of exposure) but not compared to each other, hallmarking two intrinsically different coping types of cells regarding plasma cytotoxicity.

CITED BY
  1. Bekeschus Sander, Iseni Sylvain, Reuter Stephan, Masur Kai, Weltmann Klaus-Dieter, Nitrogen Shielding of an Argon Plasma Jet and Its Effects on Human Immune Cells, IEEE Transactions on Plasma Science, 43, 3, 2015. Crossref

  2. Haertel Beate, Woedtke Thomas von, Weltmann Klaus-Dieter, Lindequist Ulrike, Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing, Biomolecules & Therapeutics, 22, 6, 2014. Crossref

  3. Karrer S., Arndt S., Plasmamedizin in der Dermatologie, Der Hautarzt, 66, 11, 2015. Crossref

  4. Bekeschus Sander, Schmidt Anke, Bethge Lydia, Masur Kai, von Woedtke Thomas, Hasse Sybille, Wende Kristian, Redox Stimulation of Human THP-1 Monocytes in Response to Cold Physical Plasma, Oxidative Medicine and Cellular Longevity, 2016, 2016. Crossref

  5. Shimizu K, Tran N A, Hayashida K, Blajan M, Comparison of atmospheric microplasma and plasma jet irradiation for increasing of skin permeability, Journal of Physics D: Applied Physics, 49, 31, 2016. Crossref

  6. Kramer Axel, Bekeschus Sander, Matthes Rutger, Bender Claudia, Stope Matthias B., Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus-Dieter, Schauer Frieder, Cold Physical Plasmas in the Field of Hygiene-Relevance, Significance, and Future Applications, Plasma Processes and Polymers, 12, 12, 2015. Crossref

  7. Schmidt Anke, von Woedtke Thomas, Bekeschus Sander, Periodic Exposure of Keratinocytes to Cold Physical Plasma: AnIn VitroModel for Redox-Related Diseases of the Skin, Oxidative Medicine and Cellular Longevity, 2016, 2016. Crossref

  8. Bazaka Kateryna, Grant Daniel S., Alancherry Surjith, Jacob Mohan V., Plasma-Assisted Fabrication and Processing of Biomaterials, in Biomedical Applications of Polymeric Materials and Composites, 2016. Crossref

  9. Schmidt Anke, Rödder Katrin, Hasse Sybille, Masur Kai, Toups Lena, Lillig Christopher H., von Woedtke Thomas, Wende Kristian, Bekeschus Sander, Redox-regulation of activator protein 1 family members in blood cancer cell lines exposed to cold physical plasma-treated medium, Plasma Processes and Polymers, 13, 12, 2016. Crossref

  10. Kramer Axel, Matthes Rutger, Bekeschus Sander, Bender Claudia, Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus Dieter, Aktueller und perspektivischer Einsatz kalter Plasmen aus hygienischer Indikation, in Plasmamedizin, 2016. Crossref

  11. Shimizu Kazuo, Krištof Jaroslav, Enhancement of Percutaneous Absorption on Skin by Plasma Drug Delivery Method, in Advanced Technology for Delivering Therapeutics, 2017. Crossref

  12. Bekeschus Sander, Lin Abraham, Fridman Alexander, Wende Kristian, Weltmann Klaus-Dieter, Miller Vandana, A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions, Plasma Chemistry and Plasma Processing, 38, 1, 2018. Crossref

  13. Bekeschus Sander, Winterbourn Christine C., Kolata Julia, Masur Kai, Hasse Sybille, Bröker Barbara M., Parker Heather A., Neutrophil extracellular trap formation is elicited in response to cold physical plasma, Journal of Leukocyte Biology, 100, 4, 2016. Crossref

  14. Crestale Letizia, Laurita Romolo, Liguori Anna, Stancampiano Augusto, Talmon Maria, Bisag Alina, Gherardi Matteo, Amoruso Angela, Colombo Vittorio, Fresu Luigia, Cold Atmospheric Pressure Plasma Treatment Modulates Human Monocytes/Macrophages Responsiveness, Plasma, 1, 2, 2018. Crossref

  15. Bekeschus Sander, Seebauer Christian, Wende Kristian, Schmidt Anke, Physical plasma and leukocytes – immune or reactive?, Biological Chemistry, 400, 1, 2018. Crossref

  16. Bekeschus Sander, Pouvesle Jean-Michel, Fridman Alexander, Miller Vandana, Cancer Immunology, in Comprehensive Clinical Plasma Medicine, 2018. Crossref

  17. Bekeschus Sander, Schmidt Anke, Kramer Axel, Metelmann Hans-Robert, Adler Frank, von Woedtke Thomas, Niessner Felix, Weltmann Klaus-Dieter, Wende Kristian, High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma, Environmental and Molecular Mutagenesis, 59, 4, 2018. Crossref

  18. Bekeschus Sander, Schütz Clarissa S., Nießner Felix, Wende Kristian, Weltmann Klaus-Dieter, Gelbrich Nadine, von Woedtke Thomas, Schmidt Anke, Stope Matthias B., Elevated H2AX Phosphorylation Observed with kINPen Plasma Treatment Is Not Caused by ROS-Mediated DNA Damage but Is the Consequence of Apoptosis, Oxidative Medicine and Cellular Longevity, 2019, 2019. Crossref

  19. Park Jeongyeon, Suh Donghwa, Tang Tianyu, Lee Hae June, Roe Jae-Seok, Kim Gyoo Cheon, Han Sungbum, Song Kiwon, Non-thermal atmospheric pressure plasma induces epigenetic modifications that activate the expression of various cytokines and growth factors in human mesoderm-derived stem cells, Free Radical Biology and Medicine, 148, 2020. Crossref

  20. Rödder Katrin, Moritz Juliane, Miller Vandana, Weltmann Klaus-Dieter, Metelmann Hans-Robert, Gandhirajan Rajesh, Bekeschus Sander, Activation of Murine Immune Cells upon Co-culture with Plasma-treated B16F10 Melanoma Cells, Applied Sciences, 9, 4, 2019. Crossref

  21. Moritz Juliane, Metelmann Hans-Robert, Bekeschus Sander, Physical Plasma Treatment of Eight Human Cancer Cell Lines Demarcates Upregulation of CD112 as a Common Immunomodulatory Response Element, IEEE Transactions on Radiation and Plasma Medical Sciences, 4, 3, 2020. Crossref

  22. Witzke Katharina, Seebauer Christian, Jesse Katja, Kwiatek Elisa, Berner Julia, Semmler Marie‐Luise, Boeckmann Lars, Emmert Steffen, Weltmann Klaus‐Dieter, Metelmann Hans‐Robert, Bekeschus Sander, Plasma medical oncology: Immunological interpretation of head and neck squamous cell carcinoma, Plasma Processes and Polymers, 17, 10, 2020. Crossref

  23. Semmler Marie Luise, Bekeschus Sander, Schäfer Mirijam, Bernhardt Thoralf, Fischer Tobias, Witzke Katharina, Seebauer Christian, Rebl Henrike, Grambow Eberhard, Vollmar Brigitte, Nebe J. Barbara, Metelmann Hans-Robert, Woedtke Thomas von, Emmert Steffen, Boeckmann Lars, Molecular Mechanisms of the Efficacy of Cold Atmospheric Pressure Plasma (CAP) in Cancer Treatment, Cancers, 12, 2, 2020. Crossref

  24. Xin Yue, Wen Xiang, Hamblin Michael R., Jiang Xian, Transdermal delivery of topical lidocaine in a mouse model is enhanced by treatment with cold atmospheric plasma, Journal of Cosmetic Dermatology, 20, 2, 2021. Crossref

  25. Bekeschus Sander, Bauer Georg, Miller Vandana, Immunology in Plasma Cancer Treatment, in Plasma Cancer Therapy, 115, 2020. Crossref

  26. Mohamed Hager, Clemen Ramona, Freund Eric, Lackmann Jan-Wilm, Wende Kristian, Connors Jennifer, Haddad Elias K., Dampier Will, Wigdahl Brian, Miller Vandana, Bekeschus Sander, Krebs Fred C., Kashanchi Fatah, Non-thermal plasma modulates cellular markers associated with immunogenicity in a model of latent HIV-1 infection, PLOS ONE, 16, 3, 2021. Crossref

  27. Xin Yue, Wen Xiang, Jiang Xian, Analgesic effect of topical lidocaine is enhanced by cold atmospheric plasma pretreatment in facial CO 2 laser treatments , Journal of Cosmetic Dermatology, 20, 9, 2021. Crossref

  28. Kupke Laura S., Arndt Stephanie, Lenzer Simon, Metz Sophia, Unger Petra, Zimmermann Julia L., Bosserhoff Anja-Katrin, Gruber Michael, Karrer Sigrid, Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro, Biomolecules, 11, 6, 2021. Crossref

  29. Bekeschus Sander, Iseni Sylvain, Luttjohann Paul, Weltmann Klaus-Dieter, Tailored Power of an RF Plasma Jet With Admixture of Nitrogen or Oxygen and Its Effects on Human Immune Cells, IEEE Transactions on Plasma Science, 49, 11, 2021. Crossref

  30. Freund Eric, Bekeschus Sander, Gas Plasma-Oxidized Liquids for Cancer Treatment: Preclinical Relevance, Immuno-Oncology, and Clinical Obstacles, IEEE Transactions on Radiation and Plasma Medical Sciences, 5, 6, 2021. Crossref

  31. Kim Han-Jun, Choi Eun-Wook, Choi Eun-Ji, Kim Hyo-Sung, Kim Junggil, Cho Guangsup, Kim Heesu, Na Seulgi, Shin Jae Ho, Do Sun Hee, Park Bong Joo, Non-thermal plasma promotes hair growth by improving the inter-follicular macroenvironment, RSC Advances, 11, 45, 2021. Crossref

  32. Adhikari Manish, Adhikari Bhawana, Adhikari Anupriya, Yan Dayun, Soni Vikas, Sherman Jonathan H., Keidar Michael, Combination drug delivery using cold atmospheric plasma technology, in Nanocarriers for the Delivery of Combination Drugs, 2021. Crossref

  33. Seebauer Christian, Freund Eric, Hasse Sybille, Miller Vandana, Segebarth Maria, Lucas Christian, Kindler Stefan, Dieke Tobias, Metelmann Hans‐Robert, Daeschlein Georg, Jesse Katja, Weltmann Klaus‐Dieter, Bekeschus Sander, Effects of cold physical plasma on oral lichen planus: An in vitro study ( Effects of CAP on OLP ) , Oral Diseases, 27, 7, 2021. Crossref

  34. Bekeschus Sander, Meyer Dorothee, Arlt Kevin, von Woedtke Thomas, Miebach Lea, Freund Eric, Clemen Ramona, Argon Plasma Exposure Augments Costimulatory Ligands and Cytokine Release in Human Monocyte-Derived Dendritic Cells, International Journal of Molecular Sciences, 22, 7, 2021. Crossref

  35. Faramarzi Fatemeh, Zafari Parisa, Alimohammadi Mina, Moonesi Mohammadreza, Rafiei Alireza, Bekeschus Sander, Szewczyk-Golec Karolina, Cold Physical Plasma in Cancer Therapy: Mechanisms, Signaling, and Immunity, Oxidative Medicine and Cellular Longevity, 2021, 2021. Crossref

  36. Min Tianhao, Xie Xin, Ren Kaijie, Sun Tuanhe, Wang Haonan, Dang Chengxue, Zhang Hao, Therapeutic Effects of Cold Atmospheric Plasma on Solid Tumor, Frontiers in Medicine, 9, 2022. Crossref

  37. Bekeschus Sander, Scherwietes Lukas, Freund Eric, Liedtke Kim Rouven, Hackbarth Christine, von Woedtke Thomas, Partecke Lars-Ivo, Plasma-treated medium tunes the inflammatory profile in murine bone marrow-derived macrophages, Clinical Plasma Medicine, 11, 2018. Crossref

  38. Bekeschus Sander, Moritz Juliane, Schmidt Anke, Wende Kristian, Redox regulation of leukocyte-derived microparticle release and protein content in response to cold physical plasma-derived oxidants, Clinical Plasma Medicine, 7-8, 2017. Crossref

  39. Schmidt Anke, Bekeschus Sander, von Woedtke Thomas, Hasse Sybille, Cell migration and adhesion of a human melanoma cell line is decreased by cold plasma treatment, Clinical Plasma Medicine, 3, 1, 2015. Crossref

  40. Bekeschus Sander, Schmidt Anke, Weltmann Klaus-Dieter, von Woedtke Thomas, The plasma jet kINPen – A powerful tool for wound healing, Clinical Plasma Medicine, 4, 1, 2016. Crossref

  41. Trimukhe Ajinkya M., Pandiyaraj K. N., Patekar Mukesh, Miller Vandana, Deshmukh Rajendra R., Perspectives and Advances of Nonthermal Plasma Technology in Cancers, IEEE Transactions on Plasma Science, 50, 8, 2022. Crossref

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