Published 6 issues per year
ISSN Print: 1045-4403
ISSN Online: 2162-6502
Indexed in
Polymeric Gene Carriers
ABSTRACT
Polymeric gene carriers are a potential alternative to using viral vectors. Polymeric carriers have relatively low immunogenicity and cytotoxicity. In addition, polymeric carriers can accommodate large-size DNA, be conjugated with appropriate functionalities, and be administered repeatedly. In spite of these advantages, polymeric gene carriers have some limitations, such as low gene transfection efficiencies and relatively short duration of gene expression. Therefore, extensive research has been conducted toward the development of efficient polymeric carriers. In this review, we discuss current problems associated with polymeric gene carriers and various strategies against transfection barriersin particular, gene stabilization and protection, cellular targeting, endosomal escaping, nuclear targeting, unpackaging, and biocompatibility. Finally, requirements for future polymeric gene carriers are considered. With all these ongoing efforts, polymeric carriers have become one of the promising gene delivery methods for human gene therapy.
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Kang Han Chang, Bae You Han, Transfection of Rat Pancreatic Islet Tissue by Polymeric Gene Vectors, Diabetes Technology & Therapeutics, 11, 7, 2009. Crossref
-
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-
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-
Samsonova Olga, Glinca Serghei, Biela Adam, Pfeiffer Christian, Dayyoub Eyas, Sahin Damla, Klebe Gerhard, Kissel Thomas, The use of isothermal titration calorimetry and molecular dynamics to show variability in DNA transfection performance, Acta Biomaterialia, 9, 2, 2013. Crossref
-
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-
Mishra Deepa, Kang Han Chang, Bae You Han, Reconstitutable charged polymeric (PLGA)2-b-PEI micelles for gene therapeutics delivery, Biomaterials, 32, 15, 2011. Crossref
-
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-
Kang H. C., Bae Y. H., pH-Tunable Endosomolytic Oligomers for Enhanced Nucleic Acid Delivery, Advanced Functional Materials, 17, 8, 2007. Crossref
-
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-
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-
Beloor Jagadish, Choi Chang Seon, Nam Hye Yeong, Park Minsun, Kim Sung Hwa, Jackson Andrew, Lee Kuen Yong, Kim Sung Wan, Kumar Priti, Lee Sang-Kyung, Arginine-engrafted biodegradable polymer for the systemic delivery of therapeutic siRNA, Biomaterials, 33, 5, 2012. Crossref
-
Kang Han Chang, Bae You Han, Polymeric Gene Transfection on Insulin-Secreting Cells: Sulfonylurea Receptor-Mediation and Transfection Medium Effect, Pharmaceutical Research, 23, 8, 2006. Crossref
-
Kang Han Chang, Samsonova Olga, Kang Sun-Woong, Bae You Han, The effect of environmental pH on polymeric transfection efficiency, Biomaterials, 33, 5, 2012. Crossref
-
Kim Hyunjung, Bae Yun Mi, Kim Hyun Ah, Hyun Hyesun, Yu Gwang Sig, Choi Joon Sig, Lee Minhyung, Synthesis and characterization of dexamethasone-conjugated linear polyethylenimine as a gene carrier, Journal of Cellular Biochemistry, 110, 3, 2010. Crossref
-
Han Jee Seung, Kim Hyun Ah, Lee Sanghyun, Lee Minhyung, VEGF receptor binding peptide-linked high mobility box group-1 box A as a targeting gene carrier for hypoxic endothelial cells, Journal of Cellular Biochemistry, 110, 5, 2010. Crossref
-
Kariduraganavar Mahadevappa Y., Kittur Arjumand A., Kamble Ravindra R., Polymer Synthesis and Processing, in Natural and Synthetic Biomedical Polymers, 2014. Crossref
-
Lee Minhyung, Choi Joon Sig, Ko Kyung Soo, Mitochondria targeting delivery of nucleic acids, Expert Opinion on Drug Delivery, 5, 8, 2008. Crossref
-
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-
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-
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-
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-
Holladay C., Keeney M., Newland B., Mathew A., Wang W., Pandit A., A reliable method for detecting complexed DNA in vitro, Nanoscale, 2, 12, 2010. Crossref
-
Kang Han Chang, Bae You Han, Endolysosomolytically Active pH-Sensitive Polymeric Nanotechnology, in Organelle-Specific Pharmaceutical Nanotechnology, 2010. Crossref
-
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-
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-
KC Remant Bahadur, Kucharski Cezary, Uludağ Hasan, Additive nanocomplexes of cationic lipopolymers for improved non-viral gene delivery to mesenchymal stem cells, Journal of Materials Chemistry B, 3, 19, 2015. Crossref
-
Lee Jiyoung, Hyun Hyesun, Kim Hyun Ah, Lee Minhyung, Dexamethasone loaded r3v6 peptide micelles for gene delivery, Journal of Controlled Release, 152, 2011. Crossref
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-
Kang Han Chang, Kang Ho-Jung, Bae You Han, A reducible polycationic gene vector derived from thiolated low molecular weight branched polyethyleneimine linked by 2-iminothiolane, Biomaterials, 32, 4, 2011. Crossref
-
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Rosenkranz A. A., Sobolev A. S., Polyethylenimine-based polyplex nanoparticles and features of their behavior in cells and tissues, Russian Chemical Bulletin, 64, 12, 2015. Crossref
-
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-
Ongkudon Clarence M., Ho Jenny, Danquah Michael K., Mitigating the looming vaccine crisis: production and delivery of plasmid-based vaccines, Critical Reviews in Biotechnology, 31, 1, 2011. Crossref
-
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-
Mesken J., Iltzsche A., Mulac D., Langer K., Modifying plasmid-loaded HSA-nanoparticles with cell penetrating peptides – Cellular uptake and enhanced gene delivery, International Journal of Pharmaceutics, 522, 1-2, 2017. Crossref
-
Kang Han Chang, Lee Eun Seong, Na Kun, Bae You Han, Stimuli-Sensitive Nanosystems: For Drug and Gene Delivery, in Multifunctional Pharmaceutical Nanocarriers, 4, 2008. Crossref
-
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-
Chapter Physiological Stress Responsive Gene Regulation Systems for Tissue Targeting, in Targeted Delivery of Small and Macromolecular Drugs, 2010. Crossref
-
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-
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-
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-
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-
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-
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-
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-
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-
Tamboli Viral, Mishra Gyan P, Mitra Ashim K, Polymeric vectors for ocular gene delivery, Therapeutic Delivery, 2, 4, 2011. Crossref
-
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Han Sangrok, Lee Youngki, Lee Minhyung, Biomimetic cell membrane-coated DNA nanoparticles for gene delivery to glioblastoma, Journal of Controlled Release, 338, 2021. Crossref
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Kim Hyun Ah, Park Ji Hwan, Lee Sanghyun, Choi Joon Sig, Rhim Taiyoun, Lee Minhyung, Combined delivery of dexamethasone and plasmid DNA in an animal model of LPS-induced acute lung injury, Journal of Controlled Release, 156, 1, 2011. Crossref
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Rhim Taiyoun, Lee Dong Yun, Lee Minhyung, Hypoxia as a target for tissue specific gene therapy, Journal of Controlled Release, 172, 2, 2013. Crossref
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Kang Han Chang, Huh Kang Moo, Bae You Han, Polymeric nucleic acid carriers: current issues and novel design approaches, Journal of Controlled Release, 164, 3, 2012. Crossref
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Kim Hyun Ah, Rhim Taiyoun, Lee Minhyung, Regulatory systems for hypoxia-inducible gene expression in ischemic heart disease gene therapy, Advanced Drug Delivery Reviews, 63, 8, 2011. Crossref
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Lee Jiyoung, Hyun Hyesun, Kim Jinyoung, Ryu Jae Hwan, Kim Hyun Ah, Park Ji Hwan, Lee Minhyung, Dexamethasone-loaded peptide micelles for delivery of the heme oxygenase-1 gene to ischemic brain, Journal of Controlled Release, 158, 1, 2012. Crossref
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