Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
Indexed in
Immune-Competent Polysaccharides from the Submerged Cultured Mycelium of Culinary—Medicinal Mushroom Lentinus strigellus Berk. et Curt. (Agaricomycetideae)
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ABSTRACT
Two main fractions of polysaccharide SL-I and SL-II-b were purified from submerged cultured mycelium of culinary—medicinal mushroom Lentinus strigellus using hot water extraction, ethanol precipitation, DEAE-cellulose chromatography, and Sephadex G-100 filtration. Polysaccharide analysis showed that SL-I is a protein-free polysaccharide with 1.15 × 104 Da molecular weight and +63.33° optical rotation, while SL-II-b is a protein-conjugated polysaccharide with 3.88 × 103 Da molecular weight and +73.67° optical rotation. The sugar constituents of polysaccharide analysis by GC showed that both fractions are heteropolysaccharides. SL-I consists of six monosaccharides, and SL-II-b consists of three monosaccharides. IR and 1H-NMR, 13C-NMR spectra further indicated that LS-I and LS-II-b are heteropolysaccharides with a(1®2),(1®4) glucosidic bonds (LS-I), and a(1®2),(1®3),(1®4) glucosidic bonds (LS-II-b). The immune-competent examination showed that both polysaccharides could increase the spleen index, carbon clearance index, phagocytic index, and white blood cell levels of CY-treated mice.
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