Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
Indexed in
Molecular Characterization of Shiitake Medicinal Mushroom, Lentinus edodes Strains (Higher Basidiomycetes) Using RAPD and ITS Sequencing
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ABSTRACT
The molecular phylogeny in seven strains of Lentinus edodes was studied based on RAPD and their internal transcribed spacers (ITS) regions. The strains were analyzed by RAPD with 20 arbitrary primers. Fifteen primers were found efficient for the amplification of the genomic DNA. The size of the polymorphic bands were in the range of 100−1000 bp. However, the size of ITS1-2 and ITS1-4 regions varied among the strains from 278 to 575 bp and from 410 to 616 bp, respectively. The higher alignment score of the ITS 1-2 region indicated more variability in the ITS 1-4 region. Thus, on the basis of RAPD-PCR and ITS sequencing it was found that strains LeC and LeI showed a high degree of divergence from all other strains.
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Pheba L George, Venkateswara R Sripathi, Seloame T Nyaku, Govind C Sharma, Ramesh V Kantety, DNA-based identification of Lentinula edodes strains with species-specific primers, African Journal of Biotechnology, 15, 7, 2016. Crossref
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WANG Li-ning, GAO Wei, WANG Qiong-ying, QU Ji-bin, ZHANG Jin-xia, HUANG Chen-yang, Identification of commercial cultivars of Agaricus bisporus in China using genome-wide microsatellite markers, Journal of Integrative Agriculture, 18, 3, 2019. Crossref
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Aminisarte Mehrnoush, Natural Strain Observation of Button Mushroom (Agaricus bisporus) from South Africa Using 18S rDNA, Asian Journal of Plant Sciences, 21, 4, 2022. Crossref