Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
Indexed in
Antimicrobial Activity of Extracts of the Oyster Culinary Medicinal Mushroom Pleurotus ostreatus (Higher Basidiomycetes) and Identification of a New Antimicrobial Compound
ABSTRACT
Pleurotus ostreatus is an edible mushroom that also has high medicinal values. In this study, P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The freeze-dried fruiting body, broth from submerged culture, and mycelial biomass of P. ostreatus were extracted using alcohols and water as solvents. The extracts were then tested for their antimicrobial activity against the growth of fungi and bacteria. It was observed that the water extract from fruiting bodies had the strongest effect in inhibiting the growth of most fungi. The most sensitive test microfungi to the inhibition were Candida albicans, Cryptococcus humicola, and Trichosporon cutaneum, and the most sensitive test bacteria were Staphylococcus aureus followed by Escherichia coli. Water extracts from culture broth or mycelial biomass were moderately inhibitive to the growth of fungi and bacteria. The alcohol-based solvents from all samples had much less antimicrobial activity against most test microorganisms. An antimicrobial compound was purified from the water extracts of fruiting bodies with Sephadex G 100 column chromatography and characterized by infrared absorption spectrum (IR), nuclear magnetic resonance (NMR), and mass spectroscopic analysis. We have identified this compound to be 3-(2-aminopheny1thio)-3-hydroxypropanoic acid. This purified compound had a minimum inhibitory concentration of 30 µg/mL and 20 µg/mL against the growth of fungi and bacteria, respectively.
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Barseghyan Gayane S., Barazani Avner, Wasser Solomon P., Medicinal Mushrooms with Anti-Phytopathogenic and Insecticidal Properties, in Mushroom Biotechnology, 2016. Crossref
-
Appiah Theresa, Boakye Yaw Duah, Agyare Christian, Antimicrobial Activities and Time-Kill Kinetics of Extracts of Selected Ghanaian Mushrooms, Evidence-Based Complementary and Alternative Medicine, 2017, 2017. Crossref
-
Özdal Murat, Gülmez Özlem, Gür Özdal Özlem, Algur Ömer Faruk, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF MYCELIAL EXTRACTS OF DIFFERENT PLEUROTUS SPECIES, Food and Health, 2019. Crossref
-
Younis Ahmed M., Yosri Mohamed, Stewart Jennifer K., In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus, Annals of Agricultural Sciences, 64, 1, 2019. Crossref
-
Gebreyohannes Gebreselema, Nyerere Andrew, Bii Christine, Sbhatu Desta B., Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms, The Scientific World Journal, 2019, 2019. Crossref
-
El Enshasy Hesham, Agouillal Farid, Mat Zarani, Malek Roslinda Abd, Hanapi Siti Zulaiha, Leng Ong Mei, Dailin Daniel Joe, Sukmawati Dalia, Pleurotus ostreatus: A Biofactory for Lignin-Degrading Enzymes of Diverse Industrial Applications, in Recent Advancement in White Biotechnology Through Fungi, 2019. Crossref
-
Younis Ahmed M., Abdel-Aziz Marwa M., Yosri Mohamed, Evaluation of Some Biological Applications of Pleurotus citrinopileatus and Boletus edulis Fruiting Bodies, Current Pharmaceutical Biotechnology, 20, 15, 2019. Crossref
-
Varghese Ruby, Dalvi Yogesh Bharat, Lamrood Prasad Y., Shinde Bharat P., Nair C. K. K., Historical and current perspectives on therapeutic potential of higher basidiomycetes: an overview, 3 Biotech, 9, 10, 2019. Crossref
-
Al-Obaidi Jameel R., Jambari Nuzul Noorahya, Ahmad-Kamil E. I., Mycopharmaceuticals and Nutraceuticals: Promising Agents to Improve Human Well-Being and Life Quality, Journal of Fungi, 7, 7, 2021. Crossref
-
Grundner Maja, Panevska Anastasija, Sepčić Kristina, Skočaj Matej, What Can Mushroom Proteins Teach Us about Lipid Rafts?, Membranes, 11, 4, 2021. Crossref
-
Pandey Aprajita Tiwari, Pandey Ishan, Kerkar Pranali, Singh M. P., Antimicrobial activity and mycochemical profile of methanol extract from Pleurotus flabellatus, Vegetos, 34, 3, 2021. Crossref
-
Awad Mohammed, Yosri Mohamed, Abdel-Aziz Marwa M., Younis Ahmed M., Sidkey Nagwa M., Assessment of the Antibacterial Potential of Biosynthesized Silver Nanoparticles Combined with Vancomycin Against Methicillin-Resistant Staphylococcus aureus–Induced Infection in Rats, Biological Trace Element Research, 199, 11, 2021. Crossref
-
Bertéli Míria Benetati Delgado, Barros Lillian, Reis Filipa S., Ferreira Isabel C. F. R., Glamočlija Jasmina, Soković Marina, Valle Juliana Silveira do, Linde Giani Andrea, Ruiz Suelen Pereira, Colauto Nelson Barros, Antimicrobial activity, chemical composition and cytotoxicity of Lentinus crinitus basidiocarp, Food & Function, 12, 15, 2021. Crossref
-
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-
Shankar Akshay, Sharma Krishna Kant, Fungal secondary metabolites in food and pharmaceuticals in the era of multi-omics, Applied Microbiology and Biotechnology, 106, 9-10, 2022. Crossref
-
Hamad Doaa, El-Sayed Heba, Ahmed Wafaa, Sonbol Hana, Ramadan Mohammed Abdel Halim, GC-MS Analysis of Potentially Volatile Compounds of Pleurotus ostreatus Polar Extract: In vitro Antimicrobial, Cytotoxic, Immunomodulatory, and Antioxidant Activities, Frontiers in Microbiology, 13, 2022. Crossref
-
Elisashvili Vladimir, Asatiani Mikheil D., Khardziani Tamar, Rai Mahendra, Natural Antimicrobials from Basidiomycota Mushrooms, in Promising Antimicrobials from Natural Products, 2022. Crossref
-
Karaltı İskender, Eraslan Emre Cem, Sarıdoğan Beste Gizem Özbey, Akata Ilgaz, Sevindik Mustafa, Total Antioxidant, Antimicrobial, Antiproliferative Potentials and Element Contents of Wild Mushroom Candolleomyces candolleanus (Agaricomycetes) from Turkey, International Journal of Medicinal Mushrooms, 24, 12, 2022. Crossref