Publicado 4 números por año
ISSN Imprimir: 0731-8898
ISSN En Línea: 2162-6537
Indexed in
Deposition of Complement Proteins on Cells Treated by Photodynamic Therapy in Vitro
SINOPSIS
Activation of the complement system has emerged as a critical event in the response of tumors to photodynamic therapy (PDT) due to its involvement in the vascular effects as well as in the inflammatory and immune reactions observed with this treatment modality. However, the exact mechanism of PDT-induced complement activation has not been precisely characterized. The present study examines the potential of PDT at the cellular level to directly activate the complement system. Mouse tumor SCCVII cells treated by Photofrin-based PDT and post-incubated in the presence of homologous (mouse) serum were analyzed by flow cytometry for binding of complement proteins on their surface. The results show that PDT induced the fixation of complement C3 protein, probably in the form of its activated fragments, and of the terminal membrane attacks complex of complement on the treated SCCVII cells. Deposition of C3/C3 fragments on human umbilical vein endothelial cells (HUVEC) treated by Photofrin-PDT and post-incubated in the presence of human serum was also detected. Complement fixation was preferentially elevated on SCCVII cell undergoing PDT-induced apoptosis. The induction of surface expression of heat shock protein 70 (HSP70) on PDT-treated cells also triggered complement deposition, since the presence of anti-HSP70 antibodies during the post-PDT incubation blocked the anchoring of C3/C3 fragments to SCCVII cells. The fact that PDT-treated cells are recognized by the complement system as their target adds an important element for understanding the mechanism of tumor response to PDT.
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Korbelik Mladen, PDT-associated host response and its role in the therapy outcome, Lasers in Surgery and Medicine, 38, 5, 2006. Crossref
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Cecic Ivana, Sun Jinghai, Korbelik Mladen, Role of Complement Anaphylatoxin C3a in Photodynamic Therapy-elicited Engagement of Host Neutrophils and Other Immune Cells, Photochemistry and Photobiology, 82, 2, 2006. Crossref
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Cecic Ivana, Minchinton Andrew I., Korbelik Mladen, The Impact of Complement Activation on Tumor Oxygenation During Photodynamic Therapy, Photochemistry and Photobiology, 83, 5, 2007. Crossref
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Korbelik Mladen, Cecic Ivana, Complement activation cascade and its regulation: Relevance for the response of solid tumors to photodynamic therapy, Journal of Photochemistry and Photobiology B: Biology, 93, 1, 2008. Crossref
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Baglo Yan, Sousa Mirta M. L., Slupphaug Geir, Hagen Lars, Håvåg Sissel, Helander Linda, Zub Kamila A., Krokan Hans E., Gederaas Odrun A., Photodynamic therapy with hexyl aminolevulinate induces carbonylation, posttranslational modifications and changed expression of proteins in cell survival and cell death pathways, Photochemical & Photobiological Sciences, 10, 7, 2011. Crossref
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Mroz Pawel, Hamblin Michael R., The immunosuppressive side of PDT, Photochemical & Photobiological Sciences, 10, 5, 2011. Crossref
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Korbelik Mladen, Zhang Wei, Separovic Duska, Amplification of cancer cell apoptosis in photodynamic therapy-treated tumors by adjuvant ceramide analog LCL29, Lasers in Surgery and Medicine, 43, 7, 2011. Crossref
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Korbelik Mladen, Complement upregulation in photodynamic therapy-treated tumors: Role of Toll-like receptor pathway and NFκB, Cancer Letters, 281, 2, 2009. Crossref
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Mroz Pawel, Szokalska Angelika, Wu Mei X., Hamblin Michael R., Mosley R. Lee, Photodynamic Therapy of Tumors Can Lead to Development of Systemic Antigen-Specific Immune Response, PLoS ONE, 5, 12, 2010. Crossref
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Panzarini Elisa, Inguscio Valentina, Dini Luciana, Immunogenic Cell Death: Can It Be Exploited in PhotoDynamic Therapy for Cancer?, BioMed Research International, 2013, 2013. Crossref
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Helbig Doris, Simon Jan C., Paasch Uwe, Photodynamic therapy and the role of heat shock protein 70, International Journal of Hyperthermia, 27, 8, 2011. Crossref
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Korbelik Mladen, Sun Jinghai, Photodynamic therapy-generated vaccine for cancer therapy, Cancer Immunology, Immunotherapy, 55, 8, 2006. Crossref
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Asadzadeh Zahra, Safarzadeh Elham, Safaei Sahar, Baradaran Ali, Mohammadi Ali, Hajiasgharzadeh Khalil, Derakhshani Afshin, Argentiero Antonella, Silvestris Nicola, Baradaran Behzad, Current Approaches for Combination Therapy of Cancer: The Role of Immunogenic Cell Death, Cancers, 12, 4, 2020. Crossref
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Korbelik Mladen, Sun Jinghai, Cecic Ivana, Photodynamic Therapy–Induced Cell Surface Expression and Release of Heat Shock Proteins: Relevance for Tumor Response, Cancer Research, 65, 3, 2005. Crossref
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Stott Brandon, Korbelik Mladen, Activation of complement C3, C5, and C9 genes in tumors treated by photodynamic therapy, Cancer Immunology, Immunotherapy, 56, 5, 2007. Crossref
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Mroz Pawel, Vatansever Fatma, Muchowicz Angelika, Hamblin Michael R., Photodynamic Therapy of Murine Mastocytoma Induces Specific Immune Responses against the Cancer/Testis Antigen P1A, Cancer Research, 73, 21, 2013. Crossref