Publicado 4 números por año
ISSN Imprimir: 2155-014X
ISSN En Línea: 2155-0158
Uncoupling Protein Inhibitor Genipin Reduces Protective Effect of Ischemic Preconditioning
SINOPSIS
Functional significance of uncoupling proteins UCP2 and UCP3 in both the cell and the body remains unknown yet. The reports of their participation in cellular protection mechanisms underlying the phenomenon of ischemic preconditioning have appeared. The aim of this study was to clarify the role of uncoupling proteins UCP2 and UCP3 in the formation of cardioprotective effect of ischemic preconditioning. In experiments on isolated rat hearts, an increase in UCP2 and UCP3 gene expression has been shown in the heart tissues influenced by ischemic preconditioning with three 5-minute episodes of stopping the flow perfusion. Similar effects were induced by prolonged ischemia-reperfusion of the myocardium. Blockade of UCP2 activity by genipin ("Wako Inc", USA, 10−5 mol / l, perfusion of isolated hearts for 15 min) abolished the protective effect of adaptation to ischemia. It has been concluded that uncoupling proteins are actively involved in the cardioprotective effect of ischemic preconditioning.