Publicado 12 números por año
ISSN Imprimir: 1521-9437
ISSN En Línea: 1940-4344
Indexed in
Compound of Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Agaricomycetes), Induces Protective Autophagy in SPCA-1 Cells through AMPK Inhibition-Independent Blockade of the Akt/mTOR Pathway
SINOPSIS
Our previous study showed that By-1, a maleimide derivative isolated from Taiwanofungus camphoratus, could induce reactive oxygen species-triggered apoptosis and G2 cell cycle arrest through a caspase-dependent pathway and also induced protective autophagy in human lung cancer SPCA-1 cells. Here, we further examined the autophagy flux and detected related proteins by Western blot analysis and fluorescence activated cell sorting, and we sought to find the exact role and underlying pathway of autophagy in SPCA-1 cells. Our results showed that By-1 treatment activated autophagy flux in SPCA-1 cells, which further confirmed that autophagy was induced by By-1 treatment in our previous study. Autophagy activator rapamycin restored cell death from By-1 treatment (21.32%) and verified that autophagy played a protective role in By-l-treated cells. Meanwhile, By-1 treatment suppressed the Akt-mammalian target of rapamycin (mTOR) pathway and the AMP-activated protein kinase (AMPK) pathway. Taken together, these findings indicate that By-1 induced protective autophagy in SPCA-1 cells through AMPK inhibition-independent blockade of the Akt/mTOR pathway.
-
Hung Hsin-Yi, Hung Chin-Chuan, Liang Jun-Weil, Chen Chin-Fu, Chen Hung-Yi, Shieh Po-Chuen, Kuo Ping-Chung, Wu Tian-Shung, Constituents and Anti-Multidrug Resistance Activity of Taiwanofungus camphoratus on Human Cervical Cancer Cells, Molecules, 24, 20, 2019. Crossref
-
Pan Haitao, Wang Yujie, Na Kun, Wang Ying, Wang Lu, Li Zhenhao, Guo Chengjie, Guo Dandan, Wang Xingya, Autophagic flux disruption contributes to Ganoderma lucidum polysaccharide-induced apoptosis in human colorectal cancer cells via MAPK/ERK activation, Cell Death & Disease, 10, 6, 2019. Crossref