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Journal of Environmental Pathology, Toxicology and Oncology

Publication de 4  numéros par an

ISSN Imprimer: 0731-8898

ISSN En ligne: 2162-6537

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 2.4 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 2.8 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.5 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00049 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.59 SJR: 0.429 SNIP: 0.507 CiteScore™:: 3.9 H-Index: 49

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Role of anb3 Integrin Receptor in the Invasive Potential of Human Cervical Cancer (SiHa) Cells

Volume 20, Numéro 3, 2001, 11 pages
DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i3.50
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RÉSUMÉ

One of the most important cell surface receptors in tumor development is avb3. To study the role of the avb3 integrin receptor in the invasive properties of tumor cells, we used human cervical tumor cells SiHa (cell surface avb3 integrin receptor-positive) and HeLa cells (cell surface avb3 integrin receptor-negative). Cell adhesion assay showed that SiHa and HeLa cells can bind very efficiently to extracellular matrix proteins fibronectin, laminin, and collagen IV, but the binding of HeLa cells to vitronectin is very poor compared to that of SiHa cells. Comparative invasion assay demonstrated a much lower invasive potential of HeLa cells than SiHa cells. Cell surface av and b3 integrin receptor subunit assay showed the expression of avb3 integrin receptor on the SiHa cell surface, whereas the HeLa cell surface lacks functional avb3 heterodimer. The zymo-gram demonstrated a higher gelatinase/MMP-2 activity in culture medium, whole cell, and membrane extract of SiHa cells than that in HeLa cells. The avb3 integrin receptor-associated MMP-2 activity of SiHa and HeLa cells was tested in a comparative zymography that clearly showed very high gelatinase/MMP-2 activity in av mAb-immunoprecipitated fraction of SiHa cell (containing avb3 heterodimer) but not in the av mAb-immunoprecipitated fraction of HeLa cell membrane extract (containing only the b3 subunit). Immu-noblot assay of av monoclonal antibody-immunoprecipitated avb3 integrin receptor from SiHa cell membrane extract with MMP-2 monoclonal antibody demonstrated the association of MMP-2 protein with avb3 integrin receptor. We concluded that avb3 integrin receptor is one of the most important cell surface molecules regulating the invasive property of cervical tumor cells because of its associated gelatinase/MMP-2 activity. Our findings will contribute to a better understanding of the role of integrin receptors, especially of the avb3 integrin receptor, in the invasive property of cancer cells and possibly affect future therapeutic approaches to cancer invasion and metastasis.

CITÉ PAR
  1. Greco Arianna, Maggini Laura, De Cola Luisa, De Marco Rossella, Gentilucci Luca, Diagnostic Implementation of Fast and Selective Integrin-Mediated Adhesion of Cancer Cells on Functionalized Zeolite L Monolayers, Bioconjugate Chemistry, 26, 9, 2015. Crossref

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