Publication de 6 numéros par an
ISSN Imprimer: 0743-4863
ISSN En ligne: 2162-660X
Indexed in
Three- and Four-Dimensional Spheroid and FiSS Tumoroid Cultures: Platforms for Drug Discovery and Development and Translational Research
RÉSUMÉ
There have been remarkable improvements in our understanding of cancer biology. However, therapeutic improvements, with a few exceptions, have been minimal. Also, significant challenges remain in translating fundamental discoveries in cancer biology and genetics into effective drugs and cures. Traditional two-dimensional monolayer cell cultures lack predictive value, resulting in a >90% failure rate of compounds in clinical trials. A developing cancer is a symbiotic tissue consisting of cancer cells, including cancer stem cells (CSCs), and cohabitating with the components of its environment to form a tumor microenvironment (TME) niche. Throughout the process of tumorigenesis, ubiquitous autocrine and paracrine signaling between the cellular and noncellular components of the TME dictates the milieu and structure of this niche. Arising out of such interactions are the cancer cell's phenotypic characteristics, such as stemness, epithelial mesenchymal transformation (EMT), and drug resistance which in turn greatly affect the response of these cells to drug therapy. For these reasons, in order to delineate the mechanism of tumorigenesis and in the process discover drugs that will have greatest impact on tumor growth, it becomes imperative to study the cancer cell in context of its microenvironment. In the present review, we enumerate the advantages of three- and four-dimensional (3D and 4D) cell cultures and describe the various cell culture platforms that are being used to study tumorigenesis in vitro. These culture systems will not only aid in the study of tumor progression complexities in a cost-effective and rapid manner; they also are expected to facilitate the discovery and delivery of therapeutic regimens that will have more success making it to the clinic.
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Mohapatra Shyam S., Batra Surinder K., Bharadwaj Srinivas, Bouvet Michael, Cosman Bard, Goel Ajay, Jogunoori Wilma, Kelley Michael J., Mishra Lopa, Mishra Bibhuti, Mohapatra Subhra, Patel Bhaumik, Pisegna Joseph R., Raufman Jean-Pierre, Rao Shuyun, Roy Hemant, Scheuner Maren, Singh Satish, Vidyarthi Gitanjali, White Jon, Precision Medicine for CRC Patients in the Veteran Population: State-of-the-Art, Challenges and Research Directions, Digestive Diseases and Sciences, 63, 5, 2018. Crossref
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Howell Mark C., Green Ryan, Khalil Roukiah, Foran Elspeth, Quarni Waise, Nair Rajesh, Stevens Stanley, Grinchuk Aleksandr, Hanna Andrew, Mohapatra Shyam, Mohapatra Subhra, Lung cancer cells survive epidermal growth factor receptor tyrosine kinase inhibitor exposure through upregulation of cholesterol synthesis, FASEB BioAdvances, 2, 2, 2020. Crossref
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Quarni Waise, Dutta Rinku, Green Ryan, Katiri Sandhyabanu, Patel Bhaumik, Mohapatra Shyam S., Mohapatra Subhra, Mithramycin A Inhibits Colorectal Cancer Growth by Targeting Cancer Stem Cells, Scientific Reports, 9, 1, 2019. Crossref
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Xie Bailu, Teusch Nicole, Mrsny Randall, Comparison of two- and three-dimensional cancer models for assessing potential cancer therapeutics, in Biomaterials for Cancer Therapeutics, 2020. Crossref
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Lu Yi, Dai Wentao, Huang Jianhua, Chen Xuexin, Yao Yuan, A Biomimetic Glue Protein Modulates Hepatic Gene Expression, Macromolecular Bioscience, 21, 3, 2021. Crossref
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Calor Alexandra, van Weissenbruch Mirjam, Senger Stefania, Role of human gastrointestinal organoids in discovery and translational medicine, in The Intestine, 2021. Crossref