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Plasma Medicine

Publication de 4  numéros par an

ISSN Imprimer: 1947-5764

ISSN En ligne: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Atmospheric Pressure Plasma Jet Application on Human Oral Mucosa Modulates Tissue Regeneration

Volume 4, Numéro 1-4, 2014, pp. 117-129
DOI: 10.1615/PlasmaMed.2014011978
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RÉSUMÉ

With the development of plasma sources generating nonthermal plasmas at atmospheric pressure the applications broaden enormously, especially in the medical field.

While earlier applications of physical plasma on biological matter focused on inactivation of microorganisms, the latest studies have revealed a modulation of eukaryotic cells by the same type of plasma. The aim of this study was to elucidate molecular mechanisms triggered by non-thermal plasma in human oral mucosa. Ex vivo biopsies were treated directly with the cold atmospheric pressure plasma jet kinpenMED and analyzed for distinctive markers of proliferation, apoptosis, DNA damage, and differentiation. Moreover, secreted cytokines and growth factors were measured in the supernatant. These results revealed stimulating effects indicated by proliferation consolidated by secretion of VEGF. We also proved that these effects were mediated into deeper layers of the mucosa, without harming the cells on top of the treated samples. DNA damage detected as H2A.X-immunoreactivity was nearly constant between all samples.

Our study revealed for the first time that human oral mucosa can be modulated by plasma treatment. Molecular markers for both cell damage and proliferation helped to identify the mechanisms triggered by plasma treatment. Moreover, our results strengthen a safe application of the kinpenMED on human oral tissue. Further studies on diseased skin are being performed in order to proceed towards a safe introduction of plasma medicine into the clinics.

CITÉ PAR
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  3. Kramer Axel, Bekeschus Sander, Matthes Rutger, Bender Claudia, Stope Matthias B., Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus-Dieter, Schauer Frieder, Cold Physical Plasmas in the Field of Hygiene-Relevance, Significance, and Future Applications, Plasma Processes and Polymers, 12, 12, 2015. Crossref

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  8. Bekeschus Sander, Schmidt Anke, Jablonowski Helena, Bethge Lydia, Hasse Sybille, Wende Kristian, Masur Kai, von Woedtke Thomas, Weltmann Klaus-Dieter, Environmental Control of an Argon Plasma Effluent and Its Role in THP-1 Monocyte Function, IEEE Transactions on Plasma Science, 45, 12, 2017. Crossref

  9. Bekeschus Sander, Schmidt Anke, Kramer Axel, Metelmann Hans-Robert, Adler Frank, von Woedtke Thomas, Niessner Felix, Weltmann Klaus-Dieter, Wende Kristian, High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma, Environmental and Molecular Mutagenesis, 59, 4, 2018. Crossref

  10. Reuter Stephan, von Woedtke Thomas, Weltmann Klaus-Dieter, The kINPen—a review on physics and chemistry of the atmospheric pressure plasma jet and its applications, Journal of Physics D: Applied Physics, 51, 23, 2018. Crossref

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  13. Hasse Sybille, Seebauer Christian, Wende Kristian, Schmidt Anke, Metelmann Hans-Robert, von Woedtke Thomas, Bekeschus Sander, Cold Argon Plasma as Adjuvant Tumour Therapy on Progressive Head and Neck Cancer: A Preclinical Study, Applied Sciences, 9, 10, 2019. Crossref

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  17. Pekbağrıyanık Tuğba, Dadas Fadime Kaya, Enhoş Şükrü, Effects of non-thermal atmospheric pressure plasma on palatal wound healing of free gingival grafts: a randomized controlled clinical trial, Clinical Oral Investigations, 25, 11, 2021. Crossref

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  19. Schütz Clarissa S., Stope Matthias B., Bekeschus Sander, Lorenzini Antonello, H2A.X Phosphorylation in Oxidative Stress and Risk Assessment in Plasma Medicine, Oxidative Medicine and Cellular Longevity, 2021, 2021. Crossref

  20. Schmidt Anke, Bekeschus Sander, How Safe is Plasma Treatment in Clinical Applications?, in Textbook of Good Clinical Practice in Cold Plasma Therapy, 2022. Crossref

  21. Gund Madline P., Hannig Matthias, Laschke Matthias W., Lehmann Antje, Schindler Axel, Rupf Stefan, Cold atmospheric plasma does not affect the regenerative potential of the pulp in rats, Plasma Processes and Polymers, 2022. Crossref

  22. Stryczewska Henryka Danuta, Boiko Oleksandr, Applications of Plasma Produced with Electrical Discharges in Gases for Agriculture and Biomedicine, Applied Sciences, 12, 9, 2022. Crossref

  23. Bekeschus Sander, von Woedtke Thomas, Emmert Steffen, Schmidt Anke, Medical gas plasma-stimulated wound healing: Evidence and mechanisms, Redox Biology, 46, 2021. Crossref

  24. Bekeschus Sander, Miebach Lea, Pommerening Jonas, Clemen Ramona, Witzke Katharina, Biological Risk Assessment of Three Dental Composite Materials following Gas Plasma Exposure, Molecules, 27, 14, 2022. Crossref

  25. Bekeschus Sander, Schmidt Anke, Weltmann Klaus-Dieter, von Woedtke Thomas, The plasma jet kINPen – A powerful tool for wound healing, Clinical Plasma Medicine, 4, 1, 2016. Crossref

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