Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Expression and Antitumor Function of Latcripin-4 RCC1 and ANK Domain Protein on HepG2 from the Shiitake Medicinal Mushroom, Lentinus edodes C91–3 (Agaricomycetes), Transcriptome
RÉSUMÉ
The recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91–3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome. The RCC1 and ANK domains from the full-length gene were selected and inserted into the expression vector pET-32a (+) and expressed in Escherichia coli Rosetta-gami (DE3). Western blotting indicated that the protein was expressed successfully. The biological function of Latcripin-4 RCC1 and ANK domain protein on HepG2 cells was studied with the CCK-8 assay. All results demonstrated that Latcripin-4 RCC1 and ANK domain protein can inhibit the growth of human HepG2 liver cancer cells, which brings new insights to identifying antitumor proteins from medicinal food for cancer therapy.
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Rezvani Vala, Pourianfar Hamid R., Mohammadnejad Safoora, Madjid Ansari Alireza, Farahmand Leila, Anticancer potentiality and mode of action of low-carbohydrate proteins and peptides from mushrooms, Applied Microbiology and Biotechnology, 104, 16, 2020. Crossref
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Balakrishnan Biju, Liang Qi, Fenix Kevin, Tamang Bunu, Hauben Ehud, Ma Linlin, Zhang Wei, Combining the Anticancer and Immunomodulatory Effects of Astragalus and Shiitake as an Integrated Therapeutic Approach, Nutrients, 13, 8, 2021. Crossref