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International Journal of Medicinal Mushrooms

Publication de 12  numéros par an

ISSN Imprimer: 1521-9437

ISSN En ligne: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes)

Volume 21, Numéro 3, 2019, pp. 301-309
DOI: 10.1615/IntJMedMushrooms.2019030106
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RÉSUMÉ

Real-time quantitative polymerase chain reaction (qRT-PCR) has emerged as a powerful and popular tool for quantitating differences in transcriptional gene expression levels between samples. Validation of the stability of reference genes is a fundamental step before initiating qRT-PCR assays. Sparassis latifolia is an edible and medicinal fungus containing a remarkably high concentration of β-glucan, which has many biological and pharmacologic activities. S. latifolia may be a model species for studying fungal photobiology because its fruiting body formation requires more light than other fungi. However, suitable reference genes for qRT-PCR have not yet been determined. In the present study, 10 candidate reference genes in S. latifolia were evaluated and validated under different developmental stages and light conditions. To evaluate the suitability of candidate reference genes, three popular software programs (geNorm, NormFinder, and BestKeeper), along with the delta Ct method, were used to analyze these genes; the final ranking was determined using RefFinder. According to our results, Actin and GAPDH were expressed at the most stable levels under different developmental stages and light conditions.

CITÉ PAR
  1. Yang Chi, Ma Lu, Xiao Donglai, Ying Zhenghe, Jiang Xiaoling, Lin Yanquan, Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia, International Journal of Molecular Sciences, 21, 1, 2019. Crossref

  2. Wang Gangzheng, Cheng Huijiao, Li Min, Zhang Chenghua, Deng Wangqiu, Li Taihui, Selection and validation of reliable reference genes for Tolypocladium guangdongense gene expression analysis under differentially developmental stages and temperature stresses, Gene, 734, 2020. Crossref

  3. Lu Yuan-Ping, Liao Jian-Hua, Guo Zhong-Jie, Zheng Hui-Qing, Lu Ling-Fang, Cai Zhi-Xin, Zeng Zhi-Heng, Ying Zheng-He, Chen Mei-Yuan, Banche Giuliana, Selection and Evaluation of Potential Reference Genes for Quantitative Real-Time PCR in Agaricus blazei Based on Transcriptome Sequencing Data, BioMed Research International, 2021, 2021. Crossref

  4. Yang Chi, Ma Lu, Xiao Donglai, Liu Xiaoyu, Jiang Xiaoling, Lin Yanquan, Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia, Scientific Reports, 12, 1, 2022. Crossref

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