Publication de 12 numéros par an
ISSN Imprimer: 1521-9437
ISSN En ligne: 1940-4344
Indexed in
Surface Decoration of Selenium Nanoparticles by Proteins from the Culinary-Medicinal Shiitake Mushroom, Lentinus edodes (Agaricomycetes), for Enhanced Fibrinolytic Activity
RÉSUMÉ
Lentinus edodes (shiitake mushroom) has exhibited fibrinolytic activity. We synthesized and characterized selenium nanoparticles (SeNPs) using protein precipitated from the mushroom. We also investigated the fibrinolytic activity of the SeNPs. The proteins from a crude extract of L. edodes were recovered through the use of aqueous 2-phase separation, and these we used as the capping agent in SeNP biosynthesis. We characterized the SeNPs using UV-visible spectrophotometry, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), transmission electron microscopy (TEM), particle size distribution analysis, and Fourier transform infrared spectroscopy (FT-IR). The fibrinolytic capability of the SeNPs was tested through an in vitro fibrin plate assay. The UV-visible spectra showed maximal absorbance at 220 nm. FESEM images showed that the SeNPs were dispersed and did not clump. The TEM images revealed a spherical shape and average size of the SeNPs. The particle size distribution analysis confirmed the mean size of the SeNPs at 64.53 nm. A strong signal for the presence of selenium was observed in the EDX analysis. The FT-IR spectrum revealed the involvement of protein functional groups in the reduction of sel-enite. Overall, the SeNPs capped with protein from shiitake mushroom were effective as an in vitro fibrinolytic agent.
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Xu Mengmeng, Zhu Song, Wang Lingling, Wei Zhiyi, Zhao Liting, Shi Guiyang, Ding Zhongyang, Influence of Selenium Biofortification on the Growth and Bioactive Metabolites of Ganoderma lucidum, Foods, 10, 8, 2021. Crossref
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Abadi Banafshe, Hosseinalipour Shamim, Nikzad Sanaz, Pourshaikhali Sara, Fathalipour-Rayeni Hadis, Shafiei Golnaz, Adeli-Sardou Mahboubeh, Shakibaie Mojtaba, Forootanfar Hamid, Capping Agents for Selenium Nanoparticles in Biomedical Applications, Journal of Cluster Science, 2022. Crossref