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Catalysis in Green Chemistry and Engineering

Publication de 4  numéros par an

ISSN Imprimer: 2572-9896

ISSN En ligne: 2572-990X

H-Index: 2

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KINETICS AND OPTIMIZATION OF LIPASE-ASSISTED ESTERIFICATION OF ISOAMYL PROPIONATE IN SOLVENT-FREE SYSTEMS

Volume 5, Numéro 2, 2022, pp. 59-69
DOI: 10.1615/CatalGreenChemEng.2022042022
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RÉSUMÉ

The research emphasizes optimizing parameters and kinetic study associated with the enzyme-catalyzed reaction of isoamyl alcohol and propionic acid in a solvent-free system. The synthesis of ester was carried out in a 50 mL baffled glass reactor with immobilized lipase (Candida Antarctica lipase B) as a catalyst. The conversion of 95% was obtained after 10 h of reaction time, at 50°C, acid-to-alcohol molar ratio of 1:3, enzyme loading of 2.5% (w/v), molecular sieves 3 g, and agitation speed of 300 rpm. Immobilized enzyme was found to retain 75% of its initial activity and was reused for up to seven cycles. The Ping-Pong Bi-Bi model with substrate inhibition was best fitted with the initial rate data. The kinetic parameter values determined at optimized conditions are as follows: maximum rate constant (Vmax) = 0.05 mol/L/min, Michaelis-Menten constant for acid (Ka) = 0.1 mol/L, Michaelis-Menten constant for alcohol (Kb) = 1.5 mol/L, constant for acid inhibition (Kia) = 0.3 mol/L, constant for alcohol inhibition (Kib) = 1.56 mol/L.

Figures

  • General schematic of esterification of propionic acid with isoamyl alcohol
  • Effect of temperature on esterification of isoamyl propionate. Reaction conditions: lipase 2% (m/v), molar ratio of propionic
acid to isoamyl alcohol 1:2, molecular sieves 3 g, stirring speed 250 rpm.
  • Effect of the molar ratio of propionic acid to isoamyl alcohol on the esterification reaction. Reaction conditions: temperature
50C, lipase loading 2% (m/v), molecular sieves 3 g, stirring speed 250 rpm.
  • Effect of biocatalyst loading on esterification of isoamyl butyrate. Reaction conditions: temperature 50C, molar ratio of
propionic acid to isoamyl alcohol 1:3, stirring speed 250 rpm, and molecular sieves 3 g.
  • Effect of speed of agitation on esterification of isoamyl butyrate. Reaction conditions: temperature 50C, lipase loading
2.5% (m/v), molar ratio of propionic acid to isoamyl alcohol 1:3, and molecular sieves 3 g.
  • Reusability of immobilized Novozym 435 for successive cycles
  • Comparison of experimental initial rates with values predicted by different models. Reaction conditions: temperature
50C, enzyme loading 2.5% (m/w), 3 g molecular sieves, agitation speed 300 rpm.
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