Library Subscription: Guest
Journal of Long-Term Effects of Medical Implants

Published 4 issues per year

ISSN Print: 1050-6934

ISSN Online: 1940-4379

SJR: 0.184 SNIP: 0.485 CiteScore™:: 1.1 H-Index: 39

Indexed in

Impact of Metal Particles Released during Ultrasonic Scaling of Titanium Surfaces on Human Gingival Fibroblasts

Volume 33, Issue 1, 2023, pp. 9-22
DOI: 10.1615/JLongTermEffMedImplants.2022043080
Get accessGet access

ABSTRACT

Purpose: Metal particles found in tissues around dental implants have been proposed to play a pathogenic role in peri-implantitis. Ultrasonic scaling has been suggested as a mechanism by which these particles can be inadvertently released into surrounding tissues. Furthermore, risk factors like diabetes can result in exacerbation of this inflammatory condition. The current study aimed to analyze metal particles released from titanium surfaces during ultrasonic scaling and their impact on pro-inflammatory cytokine production by human gingival fibroblasts.
Methods: Metal particles generated from ultrasonic scaling of titanium discs using two different tips (metal and poly-etheretherketone tips) were characterized using scanning electron microscopy and elemental analysis. Endotoxin levels and Human gingival fibroblast viability, in the presence commercial and ultrasonically generated particles were determined. Fibroblasts, cultured in high or low glucose growth medium, were incubated with commercial titanium particles or ultrasonically generated particles in the presence or absence of interluekin-1β. Interleukin 6 and interleukin 8 production were then quantified using Enzyme linked immunosorbent assay.
Results: Analysis of particles after scaling of titanium discs showed significant levels of titanium particles. Commercial titanium particles and generated particles had no effect of fibroblast viability. Endotoxin levels of all particles were too low to stimulate HGF cells. IL-1β significantly stimulated IL-6 and IL-8 production. However, commercial, and generated particles generally had no significant effect on IL- 6 and IL-8 production.
Conclusion: Our study concluded that particles generated during ultrasonic scaling had no significant effect on viability of HGF cells and cytokine production.

REFERENCES
  1. Schwarz F, Derks J, Monje A, Wang HL. Peri-implantitis. J Clin Periodont. 2018;45:S246-S266.

  2. Mombelli A, Muller N, Cionca N. The epidemiology of peri-implantitis. Clin Oral Implants Res. 2012;23:67-76.

  3. Carcuac O, Berglundh T. Composition of human peri-implantitis and periodontitis lesions. J Dent Res. 2014;93:1083-1088.

  4. Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A. Is marginal bone loss around oral implants the result of a provoked foreign body reaction? Clin Implants Dent Relat Res. 2014;16:155-165.

  5. Mombelli A, Hashim D, Cionca N. What is the impact of titanium particles and biocorrosion on implant survival and complications? A critical review. Clin Oral Implant Res. 2018;29:37-53.

  6. Berryman Z, Bridger L, Hussaini H, Rich AM, Atieh M, Tawse-Smith A. Titanium particles: An emerging risk factor for peri-implant bone loss. Saudi Dent J. 2020;32:283-92.

  7. Pettersson M, Pettersson J, Johansson A, Molin Thoren M. Titanium release in peri-implantitis. J Oral Rehab. 2019;46:179-188.

  8. Wachi T, Shuto T, Shinohara Y, Matono Y, Makhira S. Release of titanium ions from an implant surface and their effect on cytokine production related to alveolar bone resorption. Toxicology. 2015;327:1-9.

  9. Cadosch D, Sutanto M, Chan E, Mhawi A, Gautschi OP, von Katterfeld B, Simmen HP, Filgueira L. Titanium uptake, induction of RANK-L expression, and enhanced proliferation of human T-lymphocytes. J Orthop Res. 2010;28:341-7.

  10. Nishimura K, Kato T, Ito T, Oda T, Sekine H, Yoshinari M, Yajima Y. Influence of titanium ions on cytokine levels of murine splenocytes stimulated with periodontopathic bacterial lipopolysaccharide. Int J Oral Maxillofac Implants. 2014;29:472-7.

  11. Suarez-Lopez del Amo F, Garaicoa-Pazmino C, Fretwurst T, Castilho RM, Squarize CH. Dental implants-associated release of titanium particles: A systematic review. Clin Oral Implants Res. 2018;29:1085-100.

  12. Harris WH, Schiller AL, Scholler JM, Freiberg RA, Scott R. Extensive localized bone resorption in the femur following total hip replacement. J Bone Joint Surg Am. 1976;58:612-8.

  13. Noronha Oliveira M, Schunemann WVH, Mathew MT, Henriques B, Magini RS, Teughels W, Souza JCM. Can degradation products released from dental implants affect peri-implant tissues? J Periodont Res. 2018;53:1-11.

  14. Pettersson M, Kelk P, Belibasakis GN, Bylund D, Molin Thoren M, Johansson A. Titanium ions form particles that activate and execute interleukin-1p release from lipopolysaccharide-primed macrophages. J Periodont Res. 2017;52:21-32.

  15. Wilson TG, Valderrama P, Burbano M, Blansett J, Levine R, Kessler H, Rodrigues DC. Foreign bodies associated with peri-implantitis human biopsies. J Periodont. 2015;86:9-15.

  16. Fretwurst T, Buzanich G, Nahles S, Woelber JP, Riesemeier H, Nelson K. Metal elements in tissue with dental peri-implantitis: A pilot study. Clin Oral Implants Res. 2016;27:1178-86.

  17. Olmedo DG, Paparella ML, Spielberg M, Brandizzi D, Guglielmotti MB, Cabrini RL. Oral mucosa tissue response to titanium cover screws. J Periodont. 2012;83:973-80.

  18. Flateb0 RS, H0l PJ, Leknes KN, Kosler J, Lie SA, Gjerdet NR. Mapping of titanium particles in peri-implant oral mucosa by laser ablation inductively coupled plasma mass spectrometry and high-resolution optical darkfield micros-copy. J Oral Pathol Med. 2011;40:412-20.

  19. Flatebe RS, Johannessen AC, Granningster AG, Bee OE, Gjerdet NR, Grung B, Leknes KN. Host response to titanium dental implant placement evaluated in a human oral model. J Periodont. 2006;77:1201-10.

  20. Olmedo DG, Nalli G, Verdu S, Paparella ML, Cabrini RL. Exfoliative cytology and titanium dental implants: A pilot study. J Periodont. 2013;84:78-83.

  21. Safioti LM, Kotsakis GA, Pozhitkov AE, Chung WO, Daubert DM. Increased levels of dissolved titanium are associated with peri-implantitis-a cross-sectional study. J Periodont. 2017;88:436-42.

  22. Nakagawa M, Matsuya S, Udoh K. Effects of fluoride and dissolved oxygen concentrations on the corrosion behavior of pure titanium and titanium alloys. Dent Mater J. 2002;21:83-92.

  23. Pettersson M, Pettersson J, Molin Thoren M, Johansson A. Release of titanium after insertion of dental implants with different surface characteristics-an ex vivo animal study. Acta Biomater Odontol Scand. 2017;3:63-73.

  24. Alrabeah GO, Brett P, Knowles JC, Petridis H. The effect of metal ions released from different dental implant-abutment couples on osteoblast function and secretion of bone resorbing mediators. J Dent. 2017;66:91-101.

  25. Penarrieta-Juanito G, Sordi MB, Henriques B, Dotto MER, Teughels W, Silva FS, Magini RS, Souza JCM. Surface damage of dental implant systems and ions release after exposure to fluoride and hydrogen peroxide. J Periodont Res. 2019;54:46-52.

  26. Eger M, Sterer N, Liron T, Kohavi D, Gabet Y. Scaling of titanium implants entrains inflammation-induced osteolysis. Sci Rep. 2017;7:1-11.

  27. Dongari-Bagtzoglou AI, Ebersole JL. Increased presence of interleukin 6 (IL-6) and IL-8 secreting fibroblast subpopulations in adult periodontitis. J Periodont. 1998;69:899-910.

  28. Nakashyan V, Tipton D, Karydis A, Livada R, Stein S. Vitamin D attenuation of inflammatory cytokine production by gingival fibroblasts. J Periodont Res. 2017;52: 832-41.

  29. Tipton D, Hatten A, Babu JP, Dabbous MK. Effect of glycated albumin and cranberry components on interleukin-6 and matrix metalloproteinase-3 production by human gingival fibroblasts. J Periodont Res. 2016;51:228-36.

  30. Tipton DA, Pabst MJ, Dabbous MKH. Interleukin-1B and tumor necrosis factor-a independent monocyte stimulation of fibroblast collagenase activity. J Cell Biochem. 1990;44:253-64.

  31. Elenkova M, Tipton D, Karydis A, Stein SH. Vitamin D attenuates human gingival fibroblast inflammatory cytokine production following advanced glycation end product interaction with receptors for AGE. J Periodont Res. 2019;54:154-63.

  32. Kent LW, Rahemtulla F, Hockett RD, Gilleland RC, Michalek SM. Effect of lipopolysaccharide and inflammatory cytokines on interleukin-6 production by healthy human gingival fibroblasts. Infect Immun. 1998;66:608-14.

  33. Harrel SK, Wilson TG, Pandya M, Diekwisch TGH. Titanium particles generated during ultrasonic scaling of implants. J Periodont. 2019;90:241-6.

  34. Fretwurst T, Nelson K, Tarnow DP, Wang HL, Giannobile WV. Is metal particle release associated with peri-implant bone destruction? An emerging concept. J Dent Res. 2018;97:259-65.

  35. Sato S, Kishida M, Ito K. The comparative effect of ultrasonic scalers on titanium surfaces: An in vitro study. J Periodont. 2004;75:1269-73.

  36. Happe A, Sielker S, Hanisch M, Jung S. The biologic effect of particulate titanium contaminants of dental implants on human osteoblasts and gingival fibroblasts. Int J Oral Maxillofac Implants. 2019;34:673-80.

  37. Irshad M, Scheres N, Crielaard W, Loos BG, Wismeijer D, Laine ML. Influence of titanium on in vitro fibroblast-Porphyromonas gingivalis interaction in peri-implantitis. J Clin Periodont. 2013;40:841-9.

  38. Wang X, Li Y, Feng Y, Cheng H, Li D. Macrophage polarization in aseptic bone resorption around dental implants induced by Ti particles in a murine model. J Periodont Res. 2019;54:329-38.

  39. Sanz-Martm I, Paeng K, Park H, Cha JK, Jung UW, Sanz M. Significance of implant design on the efficacy of different peri-implantitis decontamination protocols. Clin Oral Invest. 2021;25:3589-97.

  40. Unursaikhan O, Lee JS, Cha JK, Park JC, Jung UW, Kim CS, Cho KS, Choi SH. Comparative evaluation of roughness of titanium surfaces treated by different hygiene instruments. J Periodont Implant Sci. 2012;42:88-94.

CITED BY
  1. Compton Anna Claire, Abhyankar Vrushali, Stein Sidney, Tipton David, Dabbous Mustafa, Abidi Ammaar, The immunomodulatory role of phytocannabinoids in anin vitroperi-implantitis model, Journal of Dental Implant Research, 41, 4, 2022. Crossref

Forthcoming Articles

Transfer of guided emergence profile developed using customized provisional implant restoration and cementation using an abutment replica technique- A case report. Vinay Sivaswamy, Balaji Anand S D, Jacob Mathew Philip, C J Venkatakrishnan, Narasimman M, Helen Mary Abraham, Pavithra B Marijuana use is Associated with Increased Rates of Hip Dislocation and Lower Insurance Reimbursement among THA Recipients Andrew Recker, Chukwuweike Gwam, Erin Kelly, Scott Douglas, Johannes Plate Surface modification of Mg alloy AZ91D using nanopowder mixed EDM for Biodegradable Implant Alok Kumar, Abhishek Singh The history of orthopedics in the long term: from general medicine to medical specialties Pavlos Altsitzioglou, Ioannis Zafeiris, Fotini Soucacos, Pantelis Limneos, Eleftheria Souliotis, Evanthia Mitsiokapa, Theodosis Saranteas, Andreas Mavrogenis COMPARISON OF ROUGHNESS, WETTABILITY AND SCANNING ELECTRON MICROSCOPY FEATURES BETWEEN SANDBLASTED ACID-ETCHED AND OXIDIZED TITANIUM DENTAL IMPLANTS Kshitiz Chhabra, Arvina Rajasekar CORRELATION BETWEEN PRIMARY STABILITY OF DENTAL IMPLANTS AND BONE DENSITY: A RETROSPECTIVE ANALYSIS Padmaja S, Arvina Rajasekar C-Reactive Protein and Erythrocyte Sedimentation Rates after total and unicompartmental knee arthroplasty - less implant equals quicker normalization Kasım Kılıçarslan, Ömer Faruk Naldöven, Enejd Veizi, Şahan Güven, Şahin Çepni, Ahmet Fırat COMPLICATIONS FOLLOWING SHORT FEMORAL NAIL FIXATION FOR INTETROCHANTERIC HIP FRACTURES. A RETROSPECTIVE STUDY Anastasia Vasilopoulou, Antonios Trichonas, Konstantinos Palaiologos, Emmanouel Antonogiannakis, Christos Nikakis, Stavros Angelis, Spyridon Maris, Christos Grekas , Efthimios Karadimas, Alexandros Apostolopoulos FEA: Connector Designs and Pontic Stress Distribution of Fixed Partial Denture Implant-Supported Metal Framework Saja Muhsin, Enas Mohammed, Khalid Bander Design and Frequency Stability Analysis of an Adaptive Neuro-Fuzzy Inference System based Artificial Pacemaker Controller in MATLAB Nader Jafarnia Dabanloo, Asghar Dabiriaghdam, Fereidoon Nooshirvan Rahatabad, Keivan Maghooli Complications following intraosseous injections of calcium phosphate bone cement in Subchondroplasty. Christos Zachariadis, Helen Leligou, Stauros Kourkoulis, Evaggelos Magnisalis, Panayiotis Papagelopoulos, Olga Savvidou The Impact of Patient Characteristics on Outcomes of Surgically Managed Vertebral Osteomyelitis in the United States: Insights from a National Database Study Jennifer Kurowicki, Stuart Changoor, Daniel Coban, Neil Patel, Kumar Sinha, Ki Hwang, Arash Emami ASSOCIATION OF AGE, GENDER AND SITE OF IMPLANT PLACEMENT - AN INSTITUTION BASED RETROSPECTIVE STUDY Harini Sri, Nabeel Ahmed, Keerthi Shasanka
Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain