Library Subscription: Guest
International Journal of Medicinal Mushrooms

Published 12 issues per year

ISSN Print: 1521-9437

ISSN Online: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

Indexed in

The In Vitro Antibacterial and Antioxidant Potentials of Helvetia bachu (Ascomycetes): A Newly Recorded Species from Pakistan

Volume 22, Issue 10, 2020, pp. 1011-1020
DOI: 10.1615/IntJMedMushrooms.2020035700
Get accessGet access

ABSTRACT

A combination of micromorphology and phylogenetic analysis based on translation elongation factor 1-alpha (TEF-1α) revealed these specimens as Helvella bachu, the first record of this species in Pakistan after China. Moreover, the material was evaluated for antibacterial and antioxidant activities. Four strains of bacteria, i.e., Bacillus subtilis, Klebsiella aerogenes, K. pneumonia, and Staphylococcus aureus were used for antibacterial activity following Soxhlet extraction. The maximum zone of inhibition of methanolic extract of H. bachu at 30 mg/mL against B. .subtilis was 31.9 ± 1.735 mm, followed by K. aerogenes (29.33 ± 2.19 mm), K. pneumoniae (27.08 ± 4.6 mm), and S. aureus (24 ± 1.53 mm), respectively. The methanolic extract, in free radical scavenging assay, of H. bachu has shown antioxidant potential with an IC50 value of 0.4047 μg/mL while that of ascorbic acid was 0.374 μg/mL. Hence, H. bachu can serve as a source of mushroom-based nutraceuticals.

REFERENCES
  1. Mao XL. Economic fungi of China. Beijing: Science Press; 1998 (in Chinese).

  2. Hu JW, Guo CY, Gong MF, Xu JB. Study on spore ejection and collection for Helvella leucopus. Edible Fungi China. 2003;22(5):15-8.

  3. Xu JY, Hu GH, Gao XH. Wild Helvella leucopus resources in Xinjiang. Chinese Wild Plant Res. 2004;22:25-6.

  4. Meng QL, Zhang PP, Hu JW. Effects of immunoregulatory activity of polysaccharide from the Bachu-mushroom. China Poultry. 2005;9:118-20.

  5. Zeng H, Zhou ZB, Hu JW, Zhang LL. Research on chemical constituents of Helvella leucopus. Pers J Tarim Univ. 2007;19:33-7.

  6. Li CH, Zhang M, Zhang LJ, Tan Q. Critical comment on Bachu mushroom. Acta Edulis Fungi. 2012;19(4):52-4.

  7. Zhao Q, Tolgor B, Zhao YC, Yang ZL, Hyde KD. Species diversity within the Helvella crispa group (Ascomycota: Helvellaceae) in China. Phytotaxa. 2015;239(2):130-42.

  8. Sultan A, Nazir K, Ayub N. Some common species of fleshy macromycetes (Discomycetes, Gasteromycetes and Agaricales) from Margalla hills national park and adjacent areas. Pak J Bot. 2001;33(SI):709-21.

  9. Razaq A, Shahzad S, Ali H, Noor A. New reported species of macrofungi from Pakistan. Pak J Agri-Food Appl Sci. 2014;2(3):67-71.

  10. Zhu M, Xue QH, He WX, Xu WL, Wang JL, Mao DM. Study of the mushroom in Bachu County I: Nutritive component. Acta Agricult Boreali. 1998;8:77-80.

  11. Hou XJ, Wei C. Optimization of extraction process of crude polysaccharides from wild edible Bachu mushroom by response surface methodology. Carbohydr Polym. 2008;72(1):67-74.

  12. Sevindik M, Akgul H, Korkmaz AI, Sen I. Antioxidant potentials of Helvella leucomelaena and Sarcosphaera coronaria. J Bacteriol Mycol. 2018;6(1):00173.

  13. Shameem N, Kamili AN, Ahmad M, Masoodi F, Parray JA. Antimicrobial activity of crude fractions and morel compounds from wild edible mushrooms of North western Himalaya. Microb Pathog. 2017;105:356-60.

  14. Wang M, Zhao YC, Zhao QI, Zhou DQ. Helvella sublactea sp. nov. (Helvellaceae) from southwestern China. Phytotaxa. 2016;253(2):131-38.

  15. Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Urs SMN, Somasundaram R. Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem. 2006;54(26):9764-72.

  16. Morris HJ, Llaurado G, Beltran Y, Lebeque Y, Bermudez RC, Garda N, Gaime-Perraud I, Moukha S. The use of mushrooms in the development of functional foods, drugs, and nutraceuticals. In: Ferreira I, Morales P, Lillian Barros L, editors. Wild plants, mushrooms and nuts: Functional food properties and applications. Chichester (UK): Wiley & Sons; 2017. p. 123-57.

  17. Zeng D, Zhu S. Purification, characterization, antioxidant and anticancer activities of novel polysaccharides extracted from Bachu mushroom. Int J Biol Macromol. 2018;107:1086-92.

  18. Zmitrovich IV, Arefyev SP, Bondartseva MA, Belova NV, Khimich YR, Isaeva LG, Kapitonov VI, Vlasenko VA, Volobuev SV, Ezhov ON, Wasser SP. Profiles of little-known medicinal polypores: Haploporus odorus (Agaricomycetes). Int J Med Mushrooms. 2019;21(8):783-91.

  19. Alves M, Ferreira I, Dias J, Teixeira, V, Martins A, Pintado M. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Med. 2012;78(16):1707-18.

  20. Pinu F, Villas-Boas S, Aggio R. Analysis of intracellular metabolites from microorganisms: Quenching and extraction protocols. Metabolites. 2017;7(4):53-62.

  21. Gebreyohannes G, Nyerere A, Bii C, Sbhatu DB. Investigation of antioxidant and antimicrobial activities of different extracts of Auricularia and Termitomyces species of mushrooms. Sci World J. 2019;2019(3):1-10.

  22. Pem D, Hyde KD, Doilom M, Camporesi E, Hongsanan S, Rampadarath S, Jeewon R. Multigene phylogenetic analyses to establish new Valsaria species and taxonomic significance of spore ornamentation. PLoS One. 2019;14(6):e0217982.

  23. Hwang J, Zhao Q, Yang ZL, Wang Z, Townsend JP. Solving the ecological puzzle of mycorrhizal associations using data from annotated collections and environmental samples - an example of saddle fungi. Environ Microbiol Rep. 2015;7(4):658-67.

  24. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia. 2005;97:84-98.

  25. Badshah H, Ali B, Shah SA, Alam MM, Aly HI, Mumtaz AS. First record of Morchella pulchella from Pakistan. Mycotaxon. 2018;133(1):201-7.

  26. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018.35(6):1547-49.

  27. Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-8.

  28. Huson DH, Scornavacca C. Dendroscope 3: An interactive tool for rooted phylogenetic trees and networks. Syst Biol. 2012;61(6):1061-67.

  29. Ronquist F, Teslenko M, Van der Mark P, Ayres DL, Darling A, Hohna S, Huelsenbeck JP. Mr. Bayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012;61(3):539-42.

  30. Brand-Williams W, Cuvelier ME, Berset CLWT. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995;28(1):25-30.

  31. Badshah H, Ullah F, Khan MU, Mumtaz AS, Malik RN. Pharmacological activities of selected wild mushrooms in South Waziristan (FATA), Pakistan. S Afr J Bot. 2015;97:107-10.

  32. Redfern J, Verran J, Burdass D, Kinninmonth M. Using Soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. J Microbiol Biol Educ. 2014;15(1):45-46.

  33. Koneman EW. Color atlas and textbook of diagnostic microbiology. 3rd ed. Philadelphia: Lippincott; 1988.

  34. Skrede I, Carlsen T, Schumacher T. A synopsis of the saddle fungi (Helvella: Ascomycota) in Europe - species delimitation, taxonomy and typification. Persoonia. 2017;39:201-53.

  35. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A. 2012;109(16):6241-46.

  36. Tekpinar AD, Kalmer A. Utility of various molecular markers in fungal identification and phylogeny. Nova Hedwigia. 2019;109(1-2):187-224.

  37. Otieno OD, Onyango C, Onguso JM, Matasyoh LG, Wanjala BW, Wamalwa M, Harvey JJW. Genetic diversity of Kenyan native oyster mushroom (Pleurotus). Mycologia. 2015;107(1):32-38.

  38. Kalaw S. Albinto R. Functional activities of Philippine wild strain of Coprinus comatus (O.F. Mull.: Fr.) Pers. and Pleurotus cystidiosus OK Miller grown on rice straw-based substrate formulation. Mycosphere. 2014;5(5):646-55.

  39. Badshah H, Qureshi RA, Khan J, Ullah F, Fahad S, Ullah F, Khan N. Pharmacological screening of Morchella esculenta (L.) Pers., Calvatia gigantea (Batsch ex Pers.) Lloyd and Astraeus hygrometricus Pers., mushroom collected from South Waziristan (FATA). J Med Plant Res. 2012;10:1853-59.

  40. Dundar A, Veysi O, Sadin Ozdemir, Kadir SC, Mehmet B, Eren O, Gul O, Abdunnasir Y. Antioxidant, antimicrobial, cytotoxic and anticholinesterase activities of seven mushroom species with their phenolic acid composition. J Horticult. 2015;2(4):1000161.

  41. Abdullah N, Haimi MZD, Lau BF, Annuar MSM. Domestication of a wild medicinal sclerotial mushroom, Lignosus rhinocerotis (Cooke) Ryvarden. Ind Crops Prod. 2013; 47:256-61.

  42. Liu K, Wang J, Zhao L, Wang Q. Anticancer, antioxidant and antibiotic activities of mushroom Ramaria flava. Food Chem Toxicol. 2013;58:375-80.

CITED BY
  1. Abdullah , Khan Shujaul Mulk, Kashif Rabia Affza, Haq Zahoor Ul, Ahmad Zeeshan, Haq Amin Ul, Haq Muhammad Abdul, Ethnobotanical Appraisal of Medicinal Plants from Bajaur; A Remote Area of the Khyber Pakhtunkhwa Province of Pakistan, in Ethnobiology of Mountain Communities in Asia, 2021. Crossref

  2. Ali Shahab, Imran Amna, Fiaz Muhammad, Khalid Abdul Nasir, Khan Shujaul Mulk, Molecular identification of True Morels (Morchella spp.) from the Hindu Kush temperate forests leads to three new records from Pakistan, Gene Reports, 23, 2021. Crossref

Begell Digital Portal Begell Digital Library eBooks Journals References & Proceedings Research Collections Prices and Subscription Policies Begell House Contact Us Language English 中文 Русский Português German French Spain