Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
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Fractionation and Biological Activities of Water-Soluble Polysaccharides from Sclerotium of Tiger Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes)
ABSTRACT
This study investigated antioxidant and anti-inflammatory properties, and the direct cytotoxic effect of Lignosus rhinocerotis fractions, especially the polysaccharide fraction, on nasopharyngeal carcinoma cells. L. rhinocerotis crude extract was obtained through hot water extraction. The precipitate saturated with 30% ammonium sulfate was purified with ion-exchanged chromatography. Gel permeation chromatography multiangle laser light scattering analysis equipped with light scattering and UV signals revealed two district groups of polymers. A total of four peaks were observed in the total carbohydrate test. Fraction C, which was the second region of the second peak eluted with 0.3 M NaOH, showed the highest integrated molecular weight, whereas fraction E had the lowest integrated molecular weight of 19,790 Da. Fraction A contained the highest β-D-glucan content. Enzymatic analysis showed that most of the polysaccharide fractions contained β-1-3 and β-1-6 skeletal backbones. The peak eluted with 0.6 M NaOH was separated in fraction D (flask 89−92) and fraction E (93−96). The results showed that fraction E expressed higher antioxidant activities than fraction D whereas fraction D expressed higher chelating activity than fraction E. The extract saturated with 30% ammonium sulfate exhibited higher reducing power than the extract saturated with 100% ammonium sulfate. Fractions D and E significantly inhibited the secretion of tumor necrosis factor-α in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. There was no apparent difference in the viability of cells exposed or unexposed to L. rhinocerotis fractions.
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Wu Bingmin, Wang Yange, Zeng Lishan, Li Wengkun, An Lin, Li Jingyan, Zhan Ruoting, Kang Huasheng, Liu Li, Lin Ying, Zhang Guifang, Ahmad Aqeel, Comparative Analysis of the Metabolites and Biological Activity of Cultivated and Wild Lignosus rhinocerotis, BioMed Research International, 2022, 2022. Crossref
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Xiong Chuan, Zhu Yu, Luo Qiang, Phan Chia Wei, Huo Yujie, Li Ping, Li Qiang, Jin Xin, Huang Wenli, Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6‐hydroxydopamine‐induced apoptosis in PC12 cells by inhibiting NF‐κB activation , Food Science & Nutrition, 2022. Crossref
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Tan Yong Hui, Lim Crystale Siew Ying , Wong Kah-Hui, Sabaratnam Vikineswary , Cytoprotective Effects of the Tiger's Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) Sclerotia against Oxidative Stress in PC12 Cells , International Journal of Medicinal Mushrooms, 24, 12, 2022. Crossref