Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
IF:
1.4
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.318
SNIP:
0.415
CiteScore™:
2.6
H-Index:
41
Indexed in
Preliminary Studies on Mating and Improved Strain Selection in the Tropical Culinary-Medicinal Mushroom Lentinus squarrosulus Mont. (Agaricomycetideae)
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ABSTRACT
Lentinus squarrosulus Mont. is an important tropical edible and medicinal mushroom distributed across Sub-Saharan Africa and most parts of Asia. Intrastock mating study was performed using single spore isolates (ssi) from a wild strain (MBFBL 201). Mycelia growth and morphology characterization indicated that milled corn straw agar (CSA) medium best supports mycelia growth compared to two other media: potato dextrose agar (PDA) and yeast peptone soluble starch agar (YPSS). The results from ssi pairings and analysis confirmed a tetrapolar mating system for L. squarrosulus. Further characterization of the resulting dikaryons indicated that the growth rate of the parent monokaryons does not affect the growth rate of the resulting dikaryon. Primordial formation in the resulting dikaryons with the fastest growth rate was 12 days after inoculation. Under similar conditions, MBFBL 201 and another wild strain, MBFBL 269, produced primordia after 28 and 32 days, respectively, indicating that intrastock breeding could be an effective method for obtaining improved strains of L. squarrosulus. Further characterization to test growth and performance in order to obtain high-yield strains for use in commercial cultivation is ongoing.
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Isikhuemhen Omoanghe S., Mikiashvili Nona A., Adenipekun Clementina O., Ohimain Elijah I., Shahbazi Ghasem, The tropical white rot fungus, Lentinus squarrosulus Mont.: lignocellulolytic enzymes activities and sugar release from cornstalks under solid state fermentation, World Journal of Microbiology and Biotechnology, 28, 5, 2012. Crossref
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N. Anike Felicia, Isikhuemhen Omoanghe S., Blum Dietrich, Neda Hitoshi, Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>, Advances in Bioscience and Biotechnology, 06, 08, 2015. Crossref
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