Published 12 issues per year
ISSN Print: 1521-9437
ISSN Online: 1940-4344
Indexed in
Structure and Antitumor Activity of 1,3-b-Glucan from Cultivated Fruit Bodies of Culinary–Medicinal Mushroom Hypsizygus marmoreus (Peck) Bigel. (Agaricomycetideae)
ABSTRACT
Hypsizygus marmoreus is an edible mushroom widely cultured in Japan. Polysaccharide fractions were prepared from the cultured H. marmoreus by repeated extraction with hot water (HmHWE), cold NaOH, and then hot NaOH. The residue was further treated with NaClO and prepared extract (HmCHAE) and residue (HmCHAR). The cold NaOH extract was further separated into water-soluble (HmCAEE) and -insoluble (HmCAER) fractions. The hot NaOH extract was also separated into water-soluble (HmHAEE) and -insoluble (HmHAER) fractions. Cold and hot NaOH extracts showed antitumor activity to the solid form of Sarcoma 180 in ICR mice. By chemical, enzymic, and NMR analyses, HmCAEE and HmHAEE contained significant amounts of 6-branched 1,3-b-glucan. In addition to 6-branched 1,3-b-glucan, NaOH extracted, water-insoluble fraction contained 1,3-a-glucan. Purified 1,3-a-glucan did not show antitumor activity. These facts strongly suggested that b-glucans but not a-glucans show antitumor activity in H. marmoreus.
-
Ishibashi Ken-ichi, Miura Noriko N., Adachi Yoshiyuki, Tamura Hiroshi, Tanaka Shigenori, Ohno Naohito, The solubilization and biological activities ofAspergillusβ-(1â3)-d-glucan, FEMS Immunology & Medical Microbiology, 42, 2, 2004. Crossref
-
Yoshino K., Nishimura M., Watanabe A., Saito S., Sano M., Preventive Effects of Edible Mushroom (Hypsizigus marmoreus) on Mouse Type IV Allergy: Fluctuations of Cytokine Levels and Antioxidant Activities in Mouse Sera, Journal of Food Science, 73, 3, 2008. Crossref
-
Akavia E., Beharav A., Wasser S.P., Nevo E., Disposal of agro-industrial by-products by organic cultivation of the culinary and medicinal mushroom Hypsizygus marmoreus, Waste Management, 29, 5, 2009. Crossref
-
Tsai Pei-Feng, Ma Chia-Yu, Effect of a glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow on growth and differentiation of human leukemic U937 cells, Food and Chemical Toxicology, 62, 2013. Crossref
-
Zhang Jinjing, Chen Hui, Chen Mingjie, Wang Hong, Song Xiaoxia, Feng Zhiyong, Construction and application of a gene silencing system using a dual promoter silencing vector inHypsizygus marmoreus, Journal of Basic Microbiology, 57, 1, 2017. Crossref
-
Mleczek Mirosław, Siwulski Marek, Rzymski Piotr, Budka Anna, Kalač Pavel, Jasińska Agnieszka, Gąsecka Monika, Budzyńska Sylwia, Niedzielski Przemysław, Comparison of elemental composition of mushroom Hypsizygus marmoreus originating from commercial production and experimental cultivation, Scientia Horticulturae, 236, 2018. Crossref
-
Zhang Ying, Zeng Yan, Men Yan, Zhang Jiangang, Liu Hanmin, Sun Yuanxia, Structural characterization and immunomodulatory activity of exopolysaccharides from submerged culture of Auricularia auricula-judae, International Journal of Biological Macromolecules, 115, 2018. Crossref
-
Ohno Naohito, Fungal Polysaccharides, in Comprehensive Glycoscience, 2021. Crossref