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International Journal of Medicinal Mushrooms

Published 12 issues per year

ISSN Print: 1521-9437

ISSN Online: 1940-4344

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Structure Characterization and Anti-Inflammatory Activity of Polysaccharides from Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) by Microwave-Assisted Freeze−Thaw Extraction

Volume 24, Issue 11, 2022, pp. 49-61
DOI: 10.1615/IntJMedMushrooms.2022045268
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ABSTRACT

A water-soluble polysaccharide (GLP-2) was isolated and purified from Ganoderma lucidum by the microwave-assisted freeze−thaw method. Microwave-assisted rapid thawing treatment can greatly improve the extraction yield of polysaccharides. The chemical structure of GLP-2 was analyzed with FT-IR, HPLC, GC-MS, and NMR spectrometry. It was proved that GLP-2 is mainly composed of β-1,3-glucose and β-1,6-glucose. The chain conformation of GLP-2 was analyzed by SEC-MALLS-RI, indicating that GLP-2 adopts semi-rigid chain conformation. Its molecular weight is 16.7 × 104, and its molecular size is about 61.2 nm. MTT assay indicated that GLP-2 was non-toxic to RAW 264.7 cells in vitro. The results of anti-inflammatory studies showed that GLP-2 can inhibit the production of NO, TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophage stimulated by lipopolysaccharide (LPS). The above data showed that the extraction method used in this study can obtain a high-yield polysaccharide with anti-inflammatory activity.

REFERENCES
  1. Sui X, Guo Q, Xia Y, Cui S, Shen J, Zhang J, Ding Z. Structure features of the intracellular polysaccharide from Ganoderma lucidum and the irrelative immune-anticancer activities of GLPs. Bioact Carbohydr Diet Fibre. 2016;8(2):43-50.

  2. Zhao Y, Feng Y, Jing X, Liu Y, Liu A. Structural characterization of an alkali-soluble polysaccharide from angelica sinensis and its antitumor activity in vivo. Chem Biodivers. 2021;18(6):e2100089.

  3. Kang Q, Chen S, Li S, Wang B, Liu X, Hao L, Lu J. Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction. Int J Biol Macromol. 2019;124:1137-44.

  4. Hou C, Chen L, Yang L, Ji X. An insight into anti-inflammatory effects of natural polysaccharides. Int J Biol Macromol. 2020;153:248-55.

  5. Ren L, Zhang J, Zhang T. Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells. Food Chem. 2021;340:127933.

  6. Li J, Gu F, Cai C, Hu M, Fan L, Hao J, Yu G. Purification, structural characterization, and immunomodulatory activity of the polysaccharides from Ganoderma lucidum. Int J Biol Macromol. 2020;143:806-13.

  7. Huang SQ, Li JW, Wang Z, Pan HX, Chen JX, Ning ZX. Optimization of alkaline extraction of polysaccharides from ganoderma lucidum and their effect on immune function in mice. Molecules. 2010;15(5):3694-708.

  8. Chen TQ, Wu YB, Wu JG, Ma L, Dong ZH, Wu JZ. Efficient extraction technology of antioxidant crude polysaccharides from Ganoderma lucidum (lingzhi), ultrasonic-circulating extraction integrating with superfine-pulverization. J Taiwan Inst Chem Eng. 2014;45(1):57-62.

  9. Chuensun T, Chewonarin T, Laopajon W, Kawee-ai A, Pinpart P, Utama-ang N. Comparative evaluation of physicochemical properties of Lingzhi (Ganoderma lucidum) as affected by drying conditions and extraction methods. Int J Food Sci Technol. 2021;56(6):2751-9.

  10. Smiderle FR, Morales D, Gil-Ramirez A, de Jesus LI, Gilbert-Lopez B, Iacomini M, Soler-Rivas C. Evaluation of micro-wave-assisted and pressurized liquid extractions to obtain beta-D-glucans from mushrooms. Carbohydr Polym. 2017;156: 165-74.

  11. Hwang IW, Kim BM, Kim YC, Lee SH, Chung SK. Improvement in beta-glucan extraction from Ganoderma lucidum with high-pressure steaming and enzymatic pre-treatment. Appl Biol Chem. 2018;61(2):235-42.

  12. Lu J, He R, Sun P, Zhang F, Linhardt RJ, Zhang A. Molecular mechanisms of bioactive polysaccharides from Ganoderma lucidum (lingzhi), a review. Int J Biol Macromol. 2020;150:765-74.

  13. Liu Y, Zhang J, Tang Q, Yang Y, Guo Q, Wang Q, Wu D, Cui SW. Physicochemical characterization of a high molecular weight bioactive beta-D-glucan from the fruiting bodies of Ganoderma lucidum. Carbohydr Polym. 2014;101: 968-74.

  14. Sletmoen M, Stokke BT. Higher order structure of (1,3)-beta-D-glucans and its influence on their biological activities and complexation abilities. Biopolymers. 2008;89(4):310-21.

  15. Zhang Y, Li S, Wang X, Zhang L, Cheung PCK. Advances in lentinan: isolation, structure, chain conformation and bioactivities. Food Hydrocolloids. 2011;25(2):196-206.

  16. Meng Y, Lyu F, Xu X, Zhang L. Recent advances in chain conformation and bioactivities of triple-helix polysaccharides. Biomacromolecules. 2020;21(5):1653-77.

  17. Zheng X, Lu FZ, Xu XJ, Zhang LN. Extended chain conformation of beta-glucan and its effect on antitumor activity. J Mater Chem B. 2017;5(28):5623-31.

  18. Santos-Neves JC, Pereira MI, Carbonero ER, Gracher AHP, Alquini G, Gorin PAJ, Sassaki GL, Iacomini M. A novel branched ab-glucan isolated from the basidiocarps of the edible mushroom Pleurotus florida. Carbohydr Polym. 2008;73(2):309-14.

  19. Cordeiro LM, de Almeida CP, Iacomini M. Unusual linear polysaccharides: (1-5)-alpha-L-arabinan, (1,3)-(1,4)-alpha-D-glucan and (1,4)-beta-D-xylan from pulp of buriti (Mauritia flexuosa), an edible palm fruit from the Amazon region. Food Chem. 2015;173:141-6.

  20. Smiderle FR, Carbonero ER, Mellinger CG, Sassaki GL, Gorin PAJ, Iacomini M. Structural characterization of a polysaccharide and a P-glucan isolated from the edible mushroom Flammulina velutipes. Phytochemistry. 2006;67(19):2189-96.

  21. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28(3):350-6.

  22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.

  23. Hou XX, Liu JY, Li ZY, Chang MC, Guo M, Feng CP, Shi JY. Fruiting body polysaccharides of Hericium erinaceus induce apoptosis in human colorectal cancer cells via ROS generation mediating caspase-9-dependent signaling pathways. Food Funct. 2020;11(7):6128-38.

  24. Wang Y, Liu Y, Yu H, Zhou S, Zhang Z, Wu D, Yang MQ, Tang QJ, Zhang JS. Structural characterization and immuno-enhancing activity of a highly branched water-soluble beta-glucan from the spores of Ganoderma lucidum. Carbohydr Polym. 2017;167:337-44.

  25. Jia X, Gao M, Li M, Wu Y, Zeng Y, Xu C. Molecular characterization of two polysaccharides from phellinus vaninii ljup and their cytotoxicity to cancer cell lines. Anticancer Agents Med Chem. 2018;18(9):1356-63.

  26. Li S, Huang Y, Wang S, Xu X, Zhang L. Determination of triple helical chain conformation of P-glucan by facile and reliable triple-detector SEC. J Phys Chem B. 2014;118(3):668-75.

  27. Xu S, Xu X, Zhang L. Branching structure and chain conformation of water-soluble glucan extracted from Auricularia auricula-judae. J Agric Food Chem. 2012;60(13):3498-506.

  28. Dong Q, Wang Y, Shi L, Yao J, Li J, Ma F, Ding K. A novel water-soluble beta-D-glucan isolated from the spores of Ganoderma lucidum. Carbohydr Res. 2012;353:100-5.

  29. Hawker CJ, Lee R, Frechet JMJ. One-step synthesis of hyperbranched dendritic polyesters. J Am Chem Soc. 1991;113(12):4583-8.

  30. Li L, Song A, Yin J, Siu K, Wong W, Wu J. Anti-inflammation activity of exopolysaccharides produced by a medicinal fungus Cordyceps sinensis Cs-HK1 in cell and animal models. Int J Biol Macromol. 2020;149:1042-50.

  31. Ooi LSM, Ooi VEC, Fung MC. Induction of immunomodulatory cytokine gene expression by Ganoderma lucidum (Curt.: Fr.) P. Karst. polysaccharide in the mouse. Int J Med Mushrooms. 2001;3(2-3):27-35.

  32. Xu Y, Zhang X, Yan X, Zhang J, Wang L, Xue H, Jiang G, Ma X, Liu X. Characterization, hypolipidemic and antioxidant activities of degraded polysaccharides from Ganoderma lucidum. Int J Biol Macromol. 2019;135:706-16.

  33. Wu GJ, Shiu SM, Hsieh MC, Tsai GJ. Anti-inflammatory activity of a sulfated polysaccharide from the brown alga Sargassum cristaefolium. Food Hydrocolloids. 2016;53:16-23.

  34. Mao Y, Song A, Li L, Siu K, Yao Z, Wu J. Effects of exopolysaccharide fractions with different molecular weights and compositions on fecal microflora during in vitro fermentation. Int J Biol Macromol. 2020;144:76-84.

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