年間 4 号発行
ISSN 印刷: 0731-8898
ISSN オンライン: 2162-6537
Indexed in
Radioprotective Effect of Carvacrol Against X-Radiation−Induced Cellular Damage in Cultured Human Peripheral Blood Lymphocytes
要約
In the present study, we evaluated the radioprotective effect of carvacrol (CVC) against X-radiation−induced cellular damage in cultured human blood lymphocytes. By MTT assay, the LD50 doses of CVC and X-radiation to lymphocytes were determined to be 100 μg/ml and 4 Gy, respectively. To explore the radioprotective effect of CVC, the cultured lymphocytes were treated with 100 μg/mL of CVC 30 min prior to 4 Gy irradiation. Subsequently, the radiation-induced damage was screened by micronuclei (MN) and dicentric chromosome (DC) frequencies and comet assay. The percentage of cell death was evaluated by acridine orange/ethidium bromide (AO/EB) staining. The radiation-induced oxidative stress was estimated by assessing the changes in the levels of enzymatic antioxidants and lipid peroxidation markers. Compared with the sham control, we observed increases in MN and DC frequencies, comet attributes, % cell death, and lipid peroxidation with a concomitant decrease in the antioxidant status of the lymphocytes treated with radiation alone. Pre-treatment of lymphocytes with CVC (100 μg/mL) altered those changes mediated by radiation. These results clearly indicate that CVC may be an effective radioprotector against X-radiation. It has the ability to scavenge the free radicals produced and to protect cells from radiation-induced cell damage.
-
Smith Tyler A., Kirkpatrick Daniel R., Smith Sean, Smith Trevor K., Pearson Tate, Kailasam Aparna, Herrmann Kortney Z., Schubert Johanna, Agrawal Devendra K., Radioprotective agents to prevent cellular damage due to ionizing radiation, Journal of Translational Medicine, 15, 1, 2017. Crossref
-
Mahran Yasmen F., Badr Amira M., Aldosari Alhanouf, Bin-Zaid Raghad, Alotaibi Hind N., Romero Francisco J., Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure, Oxidative Medicine and Cellular Longevity, 2019, 2019. Crossref
-
Aliper Alexander M., Bozdaganyan Marine E., Sarkisova Viktoria A., Veviorsky Alexander P., Ozerov Ivan V., Orekhov Philipp S., Korzinkin Mikhail B., Moskalev Alexey, Zhavoronkov Alex, Osipov Andreyan N., Radioprotectors.org: an open database of known and predicted radioprotectors, Aging, 12, 15, 2020. Crossref
-
Javadi Anis, Nikhbakht Mohammad Reza, Ghasemian Yadegari Javad, Rustamzadeh Auob, Mohammadi Mohsen, Shirazinejad Alireza, Azadbakht Saleh, Abdi Zahra, In-vivo and in vitro assessments of the radioprotective potential natural and chemical compounds: a review, International Journal of Radiation Biology, 2022. Crossref
-
Putt Karson S., Du Yuhong, Fu Haian, Zhang Zhong-Yin, High-throughput screening strategies for space-based radiation countermeasure discovery, Life Sciences in Space Research, 2022. Crossref
-
Altomare Annamaria, Fiore Michele, D’Ercole Gabriele, Imperia Elena, Nicolosi Roberta Maria, Della Posta Susanna, Pasqua Gabriella, Cicala Michele, De Gara Laura, Ramella Sara, Guarino Michele Pier Luca, Protective Role of Natural Compounds under Radiation-Induced Injury, Nutrients, 14, 24, 2022. Crossref