年間 12 号発行
ISSN 印刷: 1521-9437
ISSN オンライン: 1940-4344
Indexed in
A Validated Reverse-Phase HPLC Method for Quantitative Determination of Ganoderic Acids A and B in Cultivated Strains of Ganoderma spp. (Agaricomycetes) Indigenous to India
要約
Twenty isolates of Ganoderma spp. indigenous to India were used in this study. A reverse-phase high-performance liquid chromatography (HPLC) method was used to quantify 2 of the most bioactive triterpenes, ganoderic acid A (GAA) and ganoderic acid B (GAB), among the cultivated Ganoderma spp. The HPLC analysis was performed on an Agilent 1260 Infinity HPLC system with a Zorbax C18 column, using gradient elution of acetonitrile and 0.1% acetic acid. The detection wavelength was set at 254 nm, with a flow rate of 0.6 mL/min. The method was validated according to the guidelines of International Conference on Harmonisation and produced satisfactory results. The amount of GAA varied from 827.50 to 2010.36 µg/g, whereas GAB varied from 16.64 to 916.89 µg/g. The developed method is simple, specific, accurate, and precise, and can be useful for qualitative and quantitative evaluation of ganoderic acids.
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Ramírez Ayala Wilder , Guevara Bravo Carlos Alberto , Rodríguez Espinosa Jhon-Alexander, Ácidos ganodéricos en Ganoderma sp, Ganoderma lucidum y en su sustrato agotado, Biotecnología en el Sector Agropecuario y Agroindustrial, 19, 1, 2020. Crossref
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Li Shengyun, Yuan Yaowu, Yu Chenchen, Gao Hao, Tan Jianxin, Tian Yiling, El-Demerdash Fatma M., Establishment and Application of a Method for the Determination of Ganoderic Acid A, Journal of Food Quality, 2020, 2020. Crossref
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Cai Ming, Mu Hua, Xing Haoyong, Li Zhenhao, Xu Jing, Liu Wei, Yang Kai, Sun Peilong, In vitro gastrointestinal digestion and fermentation properties of Ganoderma lucidum spore powders and their extracts, LWT, 135, 2021. Crossref
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