Publicou 4 edições por ano
ISSN Imprimir: 0731-8898
ISSN On-line: 2162-6537
IF:
2.4
5-Year IF:
2.8
Immediacy Index:
0.5
Eigenfactor:
0.00049
JCI:
0.59
SJR:
0.429
SNIP:
0.507
CiteScore™::
3.9
H-Index:
49
Indexed in
Radioprotection against DNA Damage by an Extract of Indian Green mussel, Perna viridis (L)
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RESUMO
This study describes the radioprotective ability of a hydrolysate prepared using an enzymeacid hydrolysis method from the green mussel Perna viridis in terms of its ability to prevent radiation-induced damage in plasmid DNA, cell death, reactive oxygen species (ROS) formation, and DNA damage in mice lymphocytes. The mussel hydrolysate (MH) present during irradiation showed significant protection from γ-radiation-induced strand breaks in plasmid DNA as evaluated by gel electrophoresis. Viability studies by trypan blue dye exclusion and MTT assay showed that preincubation of mice splenic lymphocytes with MH protected them from γ-radiation-mediated killing. Moreover, the presence of MH during irradiation of isolated mice lymphocytes significantly decreased the DNA damage, as measured by comet assay. Measurement of intracellular ROS by dichlorofluorescein fluorescence revealed that the presence of MH effectively reduced the ROS generated in lymphocytes by both chemical method and γ-irradiation. Prevention of DNA damage both in plasmid and lymphocytes and cell death in lymphocytes appears correlated with reduction of oxidatively generated free radicals. It is concluded that protection against radiation-induced cell death and DNA damage by MH was attributable to reduction of reactive free radical species generated by γ-radiation.
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