Publicou 4 edições por ano
ISSN Imprimir: 2155-014X
ISSN On-line: 2155-0158
H+-Ca2+ Exchanger in the Myometrium Mitochondria: Modulation by Exogenous and Endogenous Compounds
RESUMO
The properties of ΔpH-induced Ca2+-transport from isolated rat myometrium mitochondria were investigated. Ca2+-accumulation was carried out in the presence of Mg-ATP2- and succinate. Transport of Ca2+ was recorded using Ca2+-sensitive fluorescent probe Fluo-4 AM. It has been revealed that acidification of extramitochondrial medium is accompanied by stimulation of Ca2+ release from the mitochondria. This process is relatively insensitive to the tetraphenylphosphonium which is a specific inhibitor of Na+-Ca2+ exchanger of mitochondrial inner membrane, but the latter is inhibited in the presence of monoclonal antibodies directed against the protein LETM1 (Anti-LETM1). LETM1 protein in some tissues is the molecular basis of the mitochondrial H+-Ca2+ exchanger operation. It has been found that the H+-Ca2+ exchanger is stimulated by 100 μM amiloride (diuretic) and inhibited by Mg ions in milimolar concentrations. The transport system was completely resistant to the action of nitric oxide (sodium nitroprusside and sodium nitrite), but was inhibited by macrocyclic compounds of Calix [4] arenes (C-97 and C-99) in submicromolar concentrations. Thus, the mitochondria of rat myometrium are likely not to have a system of Na+-Ca2+ exchanger, while they provide the maintenance of the matrix Ca2+-homeostasis with H+-Ca2+ exchanger. The latter is stimulated with high affinity by Calix [4] arenes, which makes promising further investigations of the influence of these compounds on the Ca2+-transport process.
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