Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
A Polysaccharide from the Culinary-Medicinal Mushroom Pholiota nameko (Agaricomycetes) Inhibits the NF-κB Pathway in Dendritic Cells Through the TLR2 Receptor
RESUMO
A polysaccharide purified from Pholiota nameko (PNPS-1) was found to have anticancer and anti-inflammatory activity. This study investigated the effect of PNPS-1 on the nuclear factor (NF)-κB signaling pathway of TLR2 small interfering RNA−silenced murine bone marrow-derived dendritic cells (BMDCs) and relevant mechanisms. The expression of messenger RNA of 4 NF-κB-related genes, including MyD88, IKBKB, RelA(p65), and CCL2, was determined by real-time polymerase chain reaction; the expression of the phenotype molecule intercellular adhesion molecule-1 (ICAM-1) by flow cytometry; the protein expression of IKKβ and p65 by Western blot; the production of p65 by enzyme-linked immunosorbent assay; and the expression of p65 by immunocytochemistry. The results showed that TLR2-specific small interfering RNA could effectively inhibit the decrease in the expression of MyD88, IKBKB, CCL2, p65, and ICAM-1 in BMDCs induced by PNPS-1, and thus the transcription inactivation of NF-κB, which obviously suggests that PNPS-1 could downregulate the NF-κB signaling pathway via the TLR2 receptor.
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Li Haiping, Tao Yongqing, Zhao Pei, Zhi Dexian, Gao Xiaoxiayue, Zhao Xiaojuan, Li Mengjia, Effect of ultrasound-assisted extraction on physicochemical properties and TLR2-affinity binding of the polysaccharides from Pholiota nameko, International Journal of Biological Macromolecules, 135, 2019. Crossref
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Haiping Li, Pei Zhao, Shuhai Zhang, Dengyun Zhou, Herong Fu, Yi Su, Xinqian Wang, Protective effect of polysaccharides from Pholiota nameko on Lactobacillus casei ATCC 334 subjected to freeze-drying, LWT, 115, 2019. Crossref
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Lin His, Cheng Kuan-Chen, Lin Jer-An, Hsieh Liang-Po, Chou Chun-Hsu, Wang Yu-Ying, Lai Ping-Shan, Chu Po-Cheng, Hsieh Chang-Wei, Pholiota nameko Polysaccharides Protect against Ultraviolet A-Induced Photoaging by Regulating Matrix Metalloproteinases in Human Dermal Fibroblasts, Antioxidants, 11, 4, 2022. Crossref
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Li Haiping, Zhao Pei, Zhuo Shikang, Zhang Tongmeng, Structural characterization, free radical scavenging activity and α-glucosidase inhibitory activity of insoluble dietary fiber from Pholiota nameko, Journal of Food Measurement and Characterization, 16, 4, 2022. Crossref