Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
Indexed in
Identification and Quantification of Triterpenoids in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), with HPLC-MS/MS Methods
RESUMO
Ganoderma lucidum is one of the most commonly used mushrooms in traditional Chinese medicine, with significant immunomodulatory and antitumor effects. Triterpenoids are deemed to be the main bioactive components in G. lucidum. In this study, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (MS) and high-performance liquid chromatography-MS/MS methods were used to identify and quantify, respectively, the main triterpenoids in G. lucidum obtained from different sources. The full MS scan with a daughter ion scan was used to identify a variety of potential derivatives of triterpenoids in the mushroom. Multiple reaction monitoring was performed with transitions of m/z—515.2 → 285.1 (quantifier) and 515.2 → 497.2 (qualifier)—to determine the amount of ganoderic acid A. The developed methods were successfully used to analyze 8 G. lucidum samples from the wild and from cultivated and commercial sources. The results show that the content and composition of triterpenoids differed significantly among these samples. A total of 29 triterpenoids were explicitly or tentatively identified in the wild sample, whereas 23 triterpenoids were found in the cultured sample and 17 in the commercial sample. The results of this study provide a foundation for further research on chemical constituents of, identification of metabolites in, and quality control of the G. lucidum mushroom.
-
Chang Wei-Ting, Gao Zi-Han, Lo Yi-Ching, Wu Sheng-Nan, Evidence for Effective Inhibitory Actions on Hyperpolarization-Activated Cation Current Caused by Ganoderma Triterpenoids, the Main Active Constitutents of Ganoderma Spores, Molecules, 24, 23, 2019. Crossref
-
Wang Zhuo, Wang Hongling, Kang Zongli, Wu You, Xing Yao, Yang Yuhong, Antioxidant and anti-tumour activity of triterpenoid compounds isolated from Morchella mycelium, Archives of Microbiology, 202, 7, 2020. Crossref
-
Li Changqin, Cui Yiping, Lu Jie, Meng Lijun, Ma Changyang, Liu Zhenhua, Zhang Yan, Kang Wenyi, Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method, Food Science and Human Wellness, 10, 3, 2021. Crossref
-
Adotey Gideon, Alolga Raphael N., Quarcoo Abraham, Gedel Mohammed Ahmed, Anang Abraham K., Holliday John C., Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS)-based metabolomic analysis of mycelial biomass of three Ganoderma isolates from the Lower Volta River Basin of Ghana, Journal of Pharmaceutical and Biomedical Analysis, 205, 2021. Crossref
-
Li Baolin, Fan Shuaishuai, Hu Jingnan, Ma Yongben, Feng Yu, Wang Fengxia, Wang Xinguo, Niu Liying, Abdel Azim Nahla S., Phytochemical Analysis Using UPLC-MS/MS Combined with Network Pharmacology Methods to Explore the Biomarkers for the Quality Control of Lingguizhugan Decoction, Evidence-Based Complementary and Alternative Medicine, 2021, 2021. Crossref
-
Kiss Attila, Grünvald Petra, Ladányi Márta, Papp Viktor, Papp István, Némedi Erzsébet, Mirmazloum Iman, Heat Treatment of Reishi Medicinal Mushroom (Ganoderma lingzhi) Basidiocarp Enhanced Its β-glucan Solubility, Antioxidant Capacity and Lactogenic Properties, Foods, 10, 9, 2021. Crossref
-
Khan Mohammad Umar, Gautam Gaurav, Jan Bisma, Zahiruddin Sultan, Parveen Rabea, Ahmad Sayeed, Vitamin D from Vegetable VV Sources: Hope for the Future, Phytomedicine Plus, 2, 2, 2022. Crossref
-
Zhao Hengqiang, Lai Chang-Jiang-Sheng, Yu Yi, Wang Ya-nan, Zhao Yu-Jun, Ma Fangli, Hu Minghua, Guo Juan, Wang Xiao, Guo Lanping, Acidic hydrolysate fingerprints based on HILIC-ELSD/MS combined with multivariate analysis for investigating the quality of Ganoderma lucidum polysaccharides, International Journal of Biological Macromolecules, 163, 2020. Crossref