Publicou 12 edições por ano
ISSN Imprimir: 1521-9437
ISSN On-line: 1940-4344
IF:
1.2
5-Year IF:
1.4
Immediacy Index:
0.3
Eigenfactor:
0.00066
JCI:
0.34
SJR:
0.274
SNIP:
0.41
CiteScore™::
2.8
H-Index:
37
Indexed in
Inhibitory Effect of Ethyl Acetate Extract of the Shaggy Inc Cap Medicinal Mushroom, Coprinus comatus (Higher Basidiomycetes) Fruit Bodies on Cell Growth of Human Ovarian Cancer
Get access
RESUMO
In a previous study, we screened organic extracts of different mushroom mycelia and picked ethyl acetate extract of Coprinus comatus as one of the most active extracts against human ovarian cancer cells. In the current study, we extracted a dry powder of C. comatus fruit bodies using ethyl acetate and examined its effect on the viability of three cell lines originated from human ovarian cancer (ES-2, SKOV-3, and SW-626). This extract was active against all tested cell lines, in a dose-dependent manner (concentrations 50−200 µg/mL, P<0.01). In an attempt to segregate the active fraction, we subjected the extract to chromatography on a silica gel column. The effect of six different fractions and of the crude extract on the viability of ES-2 cells was examined after exposure time of 24 h. Fraction F (last eluted) was significantly more effective than crude extract in the reduction of cell viability (P<0.01). Fraction F was also significantly more active than crude extract in the reduction of viability of SKOV-3 cells. We next identified some of the compounds of fraction F (mainly fatty acids) by gas chromatography−mass spectrometry. In summary, ethyl acetate extract of C. comatus reduced viability of three lines of human ovarian cancer. Fractionation of this extract by a silica gel column enabled the selection of a fraction significantly more active than the original extract.
-
Zhao Shuang, Rong Cheng-Bo, Kong Chang, Liu Yu, Xu Feng, Miao Qian-Jiang, Wang Shou-Xian, Wang He-Xiang, Zhang Guo-Qing, A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of MushroomCoprinus comatus, BioMed Research International, 2014, 2014. Crossref
-
Park Ki Moon, Kwon Kyung Min, Lee Seung Ho, Evaluation of the Antioxidant Activities and Tyrosinase Inhibitory Property from Mycelium Culture Extracts, Evidence-Based Complementary and Alternative Medicine, 2015, 2015. Crossref
-
Cao Hui, Ma Shan, Guo Hong, Cui Xiaowei, Wang Shanshan, Zhong Xiaofei, Wu Yumeng, Zheng Wenxiu, Wang Haonan, Yu Jiyang, Ma Luyu, Chun-chao Han, Comparative study on the monosaccharide compositions, antioxidant and hypoglycemic activities in vitro of intracellular and extracellular polysaccharides of liquid fermented Coprinus comatus, International Journal of Biological Macromolecules, 139, 2019. Crossref
-
Nowakowski Patryk, Naliwajko Sylwia K., Markiewicz‐Żukowska Renata, Borawska Maria H., Socha Katarzyna, The two faces of Coprinus comatus —Functional properties and potential hazards , Phytotherapy Research, 34, 11, 2020. Crossref
-
Turło Jadwiga, The biotechnology of higher fungi - current state and perspectives, Folia Biologica et Oecologica, 10, 2014. Crossref
-
Nowakowski Patryk, Markiewicz-Żukowska Renata, Gromkowska-Kępka Krystyna, Naliwajko Sylwia Katarzyna, Moskwa Justyna, Bielecka Joanna, Grabia Monika, Borawska Maria, Socha Katarzyna, Mushrooms as potential therapeutic agents in the treatment of cancer: Evaluation of anti-glioma effects of Coprinus comatus, Cantharellus cibarius, Lycoperdon perlatum and Lactarius deliciosus extracts, Biomedicine & Pharmacotherapy, 133, 2021. Crossref
-
Dimopoulou Maria, Kolonas Alexandros, Mourtakos Stamatis, Androutsos Odysseas, Gortzi Olga, Nutritional Composition and Biological Properties of Sixteen Edible Mushroom Species, Applied Sciences, 12, 16, 2022. Crossref