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The influence of Cu²⁺ ions and humic acids, and also their joint action, on Daphnia magna Straus were studied in the experiments using distilled and drinking water, and also natural water taken from the Kanev reservoir. The toxicity of the aquatic environment was conditioned by the presence of free (hydrated) ions of Cu (II). It decreased with decreasing their concentration. Humic acids formed complexes with the ions of Cu (II), as a result of which the toxicity of the aquatic environment decreased. In natural waters, both dissolved organic matter and humic acids formed complexes with Cu²⁺ ions. As a consequence, these waters were characterized by the lowest toxicity. At a lower concentration of Cu²⁺ ions (50 and 100 mg/L), their almost complete detoxification was registered after 96 hours of exposure. At a higher concentration of Cu²⁺ ions, the formation of complexes occurred slowly, as a result of which the toxicity of the aquatic environment was registered for a longer time. Data on the distribution of Cu (II) in the complex compounds with humic acids in the experiments using distilled and drinking water, and also in the complexes with dissolved organic matter and humic acids in the experiments using natural water, are presented,