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ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
Indexed in
Antiproliferative and Apoptotic Activities of the Medicinal Mushroom Phellinus rimosus (Agaricomycetes) on HCT116 Human Colorectal Carcinoma Cells
Краткое описание
Phellinus rimosus is a host-specific wood-rotting polypore that has been reported to be used by some tribes in Kerala, India, for curing mumps. We isolated a novel polysaccharide-protein complex from Ph. rimosus (PPC-Pr) that possessed significant antitumor activity. In this study, we examined the antiproliferative and apoptotic properties of PPC-Pr using human colon cancer cell line HCT116 as a model system. HCT116 cells were cultured in the presence of PPC-Pr at various concentrations (100-1000 μg/mL) for 24-96 hours, and the percentage of cell viability was evaluated using a 3-(4-5 dimethylthiozol-2-yl) 2-5-diphenyl-tetrazolium bromide (MTT) assay. The results showed that PPC-Pr inhibited cell viability in a time- and concentration-dependent manner. The antiproliferative effect of PPC-Pr was associated with apoptosis on HCT116 cells. We analyzed the morphological changes in the PPC-Pr-treated HCT116 cells using fluorescent DNA-microscopy with 4',6-diamidino-2-phenylindole dihydrochloride hydrate (DAPI) and acridine orange-ethidium bromide (AO/EB) staining. With DAPI staining, the cells treated with PPC-Pr showed nuclear shrinkage and chromatin condensation. Apoptotic morphology in cellular bodies as well as chromatin condensation were also confirmed by AO/EB double staining. Oligonucleosomal DNA fragmentation in PPC-Pr-treated cells was evaluated using a comet assay. The comet assay showed that control cells had few nuclei with fragmented chromatin, whereas apoptotic nuclei were more frequent in PPC-Pr-treated cells. These investigations indicate that the polysaccharide-protein complex from Ph. rimosus possesses antiproliferative activity and induces marked apoptosis in tumor cells in vitro.
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Yu Taihen, Zhong Shi, Sun Yuqing, Sun Haiyan, Chen Weiguo, Li Yougui, Zhu Jianxun, Lu Longxi, Huo Jinxi, Aqueous extracts of Sanghuangporus vaninii induce S‑phase arrest and apoptosis in human melanoma A375 cells, Oncology Letters, 22, 2, 2021. Crossref