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ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
Indexed in
Quantification of β-Glucan from Culinary-Medicinal Mushrooms Using Novel Artificial β-Glucan Recognition Protein
Краткое описание
(1->3)-β-D-glucans (BGs), found in culinary-medicinal mushrooms, exhibit an immunostimulatory effect; hence, it is important to measure the content of BGs contained in mushrooms. BGs content in a mushroom extract was measured using a recombinant BG-binding protein, supBGRP, and compared with the existing BG assay using BGs antibody. The specificity of supBGRP enzyme immunoassay (EIA) was evaluated using a commercially available polysaccharide reagent. The supBGRP did not react to barley glucan, dextran, mannan, pustulan, and xylan, but reacted to sonifilan, and only slightly to curdlan. Among the BGs tested, supBGRP was most reactive to lentinan. The glucans were extracted using hot water and alkaline solution from the fruit body of the following edible mushrooms: Pleurotus ostreatus, Grifola frondosa, Lentinus edodes, Hypsizygus marmoreus, Flammulina velutipes, and Auricularia polytricha. All BGs extracted from edible mushrooms were detectable; in particular, the reactivity of supBGRP toward the alkaline-extracted fraction from Lentinus edodes was higher than that toward polyclonal antibody for BGs. The results suggest that supBGRP had a specific reaction to BG. The supBGRP seems to be superior to antibodies due to easy availability as a reagent and stability as a protein molecule for measurement of BGs.
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Kanno Takashi, Kim Changmin, Yamanaka Daisuke, Ishibashi Ken-ichi, Tanaka Hiroshi, Ohno Naohito, Adachi Yoshiyuki, Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test, International Journal of Molecular Sciences, 22, 4, 2021. Crossref
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Yamanaka Daisuke, Kurita Suzuka, Hanayama Yuka, Adachi Yoshiyuki, Split Enzyme-Based Biosensors for Structural Characterization of Soluble and Insoluble β-Glucans, International Journal of Molecular Sciences, 22, 4, 2021. Crossref
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Kanno Takashi, Adachi Yoshiyuki, Ohashi-Doi Katsuyo, Matsuhara Hiroki, Hiratsuka Rie, Ishibashi Ken-ichi, Yamanaka Daisuke, Ohno Naohito, Latent 1,3-β-D-glucan acts as an adjuvant for allergen-specific IgE production induced by Japanese cedar pollen exposure, Allergology International, 70, 1, 2021. Crossref