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ISSN Печать: 1521-9437
ISSN Онлайн: 1940-4344
Indexed in
Macrophages Stimulated by Polysaccharide Purified from Agaricus brasiliensis S.Wasser et al. (Agaricomycetideae) Enhance mRNA Expression of Th1 Cytokine Including IL-12 and 18
Краткое описание
Royal Sun Agaricus—Agaricus brasiliensis—has traditionally and medically been used as a health food for the prevention of cancer. We chromatographically purified an immunomodulating compound from a water-soluble fraction of A. brasiliensis. The molecular weight of this compound was determined as approximately 6200 by gel permeation chromatography. This compound enhanced the production of tumor necrosis factor (TNF)-a and nitric oxide (NO) from a murine macrophage-like cell line, RAW264.7. The result of time-course experiments on TNF-a and NO production from RAW264.7 indicated that TNF-a production was produced at least 6 hours earlier than NO production. The expression of interleukin (IL)-12 (a key role in Th1 differentiation) and IL-18 (a proinflammatory cytokine in enhancing Th1 immune response) mRNA in RAW264.7 was investigated by RT-PCR. Both mRNAs were induced 24 hours after stimulation. In conclusion, autocrine TNF-a production caused NO production from macrophage stimulated by the immunomodulating compound from A. brasiliensis. In addition, this compound might activate Th1 immune response because it increased the level of IL-12 and 18 mRNA expressions in macrophages.
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Shu Chin-Hang, Xu Chun-Jun, Lin En-Shu, Production, purification and partial characterization of a novel endo-β-1,3-glucanase from Agaricus brasiliensis, Process Biochemistry, 41, 5, 2006. Crossref
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