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International Journal of Medicinal Mushrooms
Главный редактор: Solomon P. Wasser (open in a new tab)

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ISSN Печать: 1521-9437

ISSN Онлайн: 1940-4344

The Impact Factor measures the average number of citations received in a particular year by papers published in the journal during the two preceding years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) IF: 1.2 To calculate the five year Impact Factor, citations are counted in 2017 to the previous five years and divided by the source items published in the previous five years. 2017 Journal Citation Reports (Clarivate Analytics, 2018) 5-Year IF: 1.4 The Immediacy Index is the average number of times an article is cited in the year it is published. The journal Immediacy Index indicates how quickly articles in a journal are cited. Immediacy Index: 0.3 The Eigenfactor score, developed by Jevin West and Carl Bergstrom at the University of Washington, is a rating of the total importance of a scientific journal. Journals are rated according to the number of incoming citations, with citations from highly ranked journals weighted to make a larger contribution to the eigenfactor than those from poorly ranked journals. Eigenfactor: 0.00066 The Journal Citation Indicator (JCI) is a single measurement of the field-normalized citation impact of journals in the Web of Science Core Collection across disciplines. The key words here are that the metric is normalized and cross-disciplinary. JCI: 0.34 SJR: 0.274 SNIP: 0.41 CiteScore™:: 2.8 H-Index: 37

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Phenolic Compounds Present in Medicinal Mushroom Extracts Generate Reactive Oxygen Species in Human Cells In Vitro

Том 10, Выпуск 1, 2008, pp. 1-13
DOI: 10.1615/IntJMedMushr.v10.i1.20
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Краткое описание

Hot-water extracts of the higher Basidiomycetes Agaricus bisporus (J. Lge) Imbach, A. brasiliensis S. Wasser et al., Coprinus comatus (O.F. Mull.) Pers., Ganoderma lucidum (W. Curt.: Fr.) P. Karst., and Phellinus linteus (Berk. et Curt.) Teng were made, and the resulting polysaccharide mixtures were purified by DEAE-cellulose chromatography and ethanol precipitation. The extracts were noncytopathic. A. bisporus, A. brasiliensis, and G. lucidum strongly generated reactive oxygen species (ROS) in human PBMCs and K 562 cells. C. comatus and Ph. linteus extracts had low ROS-generating capacity. In A. bisporus extract, two different classes of polysaccharides were found. The colorless polysaccharide of MW > 250 kDa caused no intracellular ROS generation; acid hydrolysis followed by HPLC analysis showed it to consist of only glucose residues, thus being a pure glucan. The light brown polyphenol/polysaccharide complex of MW 75−200 kDa was found to strongly generate ROS. ROS generation by medicinal mushrooms could therefore be due to the presence of polyphenols rather than of glucan alone. A. bisporus polysaccharide extract showed a saturation level of ROS generation at 2 mg mL−1. Generation appeared to continue in the presence of polysaccharide for more than 14 hours. Purified colorless polysaccharides of C comatus and Ph. linteus showed no ROS generation in K562 cells; G. lucidum and A. brasiliensis polysaccharides could not completely be cleaned of phenolic compounds and remained active. Competitive inhibition of ROS generation by laminarin was not observed for any of the polysaccharide extracts, suggesting that polyphenol/glucan complexes isolated from higher Basidiomycetes mushrooms are able to generate ROS without binding to a dectin receptor.

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