Acute Rat Cutaneous Wound Healing for Small and Large Wounds Using Ar/O<sub>2</sub> Atmospheric-Pressure Plasma Jet Treatment

Zhi-Hua  Lin; Kuang-Yao  Cheng; Yu-Pin  Cheng; Chen-Yon Tobias  Tschang; Hsien-Yi  Chiu; Nai-Lun  Yeh; Kou-Chi  Liao; Bi-Ren  Gu; Jong-Shinn Wu

doi:10.1615/PlasmaMed.2017020386

Plasma Medicine

Главный редактор: Satoshi Hamaguchi (open in a new tab)

Ассоциированный редактор: Gregory Fridman (open in a new tab)

Executive Editors: Klaus-Dieter Weltmann (open in a new tab) Alexander A. Fridman (open in a new tab)

Выходит 4 номеров в год

ISSN Печать: 1947-5764

ISSN Онлайн: 1947-5772

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Acute Rat Cutaneous Wound Healing for Small and Large Wounds Using Ar/O₂ Atmospheric-Pressure Plasma Jet Treatment

Том 7, Выпуск 3, 2017, pp. 227-243

DOI: 10.1615/PlasmaMed.2017020386

Submitted: May 04 2017 Accepted: May 17 2017 Published online: Jan 11 2018

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Zhi-Hua Lin
Department of Mechanical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan

Kuang-Yao Cheng
Department of Mechanical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan

Yu-Pin Cheng
Department of Dermatology, Cathay General Hospital, Taipei 106, Taiwan; Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu 300, Taiwan

Chen-Yon Tobias Tschang
Department of Mechanical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan

Hsien-Yi Chiu
Department of Dermatology, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan

Nai-Lun Yeh
Department of Department of Family Medicine, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan

Kou-Chi Liao
Department of Bio-industrial Mechatronics Engineering, National Taiwan University, Taipei 106, Taiwan

Bi-Ren Gu
Department of Mechanical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan

Jong-Shinn Wu (Corresponding author chongsin@faculty.nctu.edu.tw)
Department of Mechanical Engineering, National Chiao Tung University, Hsinchu 300, Taiwan

Краткое описание

In this study, we developed a rat model of acute wound healing treated by a cold and touchable Ar/O₂ atmospheric-pressure plasma jet (APPJ) with a jet gas temperature of < 37%C and a length of 15 mm. To generate more abundant reactive oxygen species (ROS) and reactive nitrogen species (RNS), we added various amounts of oxygen into argon. We have found that, with 0.04% oxygen addition, the APPJ can generate the most abundant ROS/ RNS such as hydroxyl radicals (OH^*), N₂^2nd+, and O atom in the jet region based on the optical emission spectroscopymeasurements. We then applied the APPJ to treat open wounds of rats directly. To demonstrate the effectiveness of this APPJ treatment for wound healing, we created two wound sizes with diameters of 8 mm (small) and 21 mm (large). The results show that APPJ treatment with 0.04% oxygen addition can greatly accelerate wound healing, especially for the group with large wounds compared with the control group without any treatment. We explain this wound-healing enhancement with plasma jet treatment using a standard histologic method, hematoxylin and eosin staining, from the sectioned skins by comparing the different experimental groups.

Ключевые слова: atmospheric pressure plasma, dielectric barrier discharge, wound healing, animal model

ЦИТИРОВАНО В

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